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Gene Expression Data
Assay Details
Assay
Reference: J:28254 Dvorak P, et al., Expression of paternal and maternal mitochondrial HSP70 family, hsc74, in preimplantation mouse embryos. Int J Dev Biol. 1995 Jun;39(3):511-7
Assay type: Immunohistochemistry
MGI Accession ID: MGI:4949195
Gene symbol: Hspa9
Gene name: heat shock protein 9
Antibody: anti-hsc74
Detection system: Secondary antibody coupled to fluorescein
Assay notes: Embryos were fixed in 95% ethanol with 1% acetic acid.
Results
Specimen 2A: embryonic day 0.5 (more )
Note: One-cell stage (10 h post ovulation).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2A
TS1: second polar body Strong 2A
Specimen 2B: embryonic day 0.7 (more )
Note: One-cell stage (18 h post ovulation).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 2B
Specimen 2C: embryonic day 1.5 (more )
Note: Two-cell stage (34 h post ovulation).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Strong 2C The cytoplasm of every blastomere or cell has significant bright signals.
Specimen 2D: embryonic day 2.0 (more )
Note: Four-cell stage (46 h post ovulation).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Strong 2D The cytoplasm of every blastomere or cell has significant bright signals.
Specimen 2E: embryonic day 2.5 (more )
Note: Eight-cell stage (58 h post ovulation).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Strong 2E The cytoplasm of every blastomere or cell has significant bright signals.
Specimen 2F: embryonic day 3.5 (more )
Note: This blastocyst is 82 h post ovulation.
Structure Level Pattern Image Note
TS4: blastocyst Strong 2F The cytoplasm of every blastomere or cell has significant bright signals.
Specimen 2G: embryonic day 0.7 (more )
Note: One-cell stage (18 h post ovulation).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent 2G
Specimen 2H: embryonic day 2.5 (more )
Note: Eight-cell stage (58 h post ovulation).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Absent 2H
Specimen 2I: embryonic day 3.5 (more )
Note: This blastocyst is 82 h post ovulation.
Structure Level Pattern Image Note
TS4: blastocyst Absent 2I
Specimen Not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: mouse Absent
Specimen 3A: embryonic day 0.5 (more )
Note: One-cell stage (10 h post ovulation).
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Absent 3A
Specimen 3B--sperm: postnatal (more )
Structure Level Pattern Image Note
TS28: germ cell of testis Present Regionally restricted 3B Expression is present in the midpiece of the sperm.
Specimen 3B--egg: postnatal (more )
Structure Level Pattern Image Note
TS28: secondary oocyte Absent 3B
Specimen 3C: embryonic day 2.0 (more )
Note: Four-cell stage (46 h post ovulation).
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Absent 3C
Specimen 3D: embryonic day 2.5 (more )
Note: Eight-cell stage (58 h post ovulation).
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Weak Regionally restricted 3D Weakly positive staining is observed in the peripheral region of the blastomeres.
Specimen 3E: embryonic day 2.7 (more )
Note: Eight-cell stage (64 h post ovulation).
Structure Level Pattern Image Note
TS3: compacted morula Strong 3E Bright staining signals were seen in every blastomere.
Specimen 3F: embryonic day 3.5 (more )
Note: This blastocyst is 82 h post ovulation.
Structure Level Pattern Image Note
TS4: inner cell mass Strong 3F Expression is present in all cells.
TS4: trophectoderm Strong 3F Expression is present in all cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory