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Gene Expression Data
Assay Details
Assay
Reference: J:105464 Amrein L, et al., Expression patterns of nm23 genes during mouse organogenesis. Cell Tissue Res. 2005 Dec;322(3):365-78
Assay type: RNA in situ
MGI Accession ID: MGI:4949069
Gene symbol: Nme4
Gene name: NME/NM23 nucleoside diphosphate kinase 4
Probe: nm23-M4 i
Probe preparation: labelled with S35
Visualized with: Autoradiography
Assay notes: Two oligonucleotide probes were described for this gene (see probe records MGI:4948922 and MGI:4948923 for the sequences). Authors indicate that one or both were used in this RNA in situ hybridization assay.
Results
Specimen 3d: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: embryo Weak 3d Expression is hardly detectable at this stage.

Specimen Table 1 E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebellum Weak
TS21: intestine Weak
TS21: trigeminal ganglion Weak
TS21: cerebral cortex Absent
TS21: dorsal root ganglion Absent
TS21: heart Absent
TS21: lung Absent
TS21: midbrain Absent
TS21: spinal cord Absent
TS21: thymus primordium Absent

Specimen Table 1 E15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cerebral cortex ventricular layer Strong
TS23: cerebellum Moderate
TS23: cortical plate Moderate
TS23: intestine Moderate
TS23: eye Weak
TS23: hair follicle Weak
TS23: heart Weak
TS23: lung Weak
TS23: midbrain Weak
TS23: dorsal root ganglion Absent
TS23: spinal cord Absent
TS23: thymus primordium Absent
TS23: tongue Absent
TS23: trigeminal ganglion Absent

Specimen Table 1 E18.5: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebellum Present Regionally restricted Expression was abundant in the external granular layer and moderate in the internal granular layer.
TS26: cerebral cortex ventricular zone Strong
TS26: heart Strong
TS26: intestine Moderate
TS26: cortical plate Weak
TS26: hair follicle Weak
TS26: midbrain Weak
TS26: thymus Weak
TS26: tongue Weak
TS26: main olfactory bulb Absent Expression was not detected in the granular cell layer and subependymal zone.
TS26: dorsal root ganglion Absent
TS26: lung Absent
TS26: spinal cord Absent
TS26: trigeminal ganglion Absent

Specimen 5d: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebral cortex ventricular zone Present 5d

Specimen 6d: embryonic day 18.5 (more )
Note: Double labeled with BrdU (brown).
Structure Level Pattern Image Note
TS26: cerebral cortex ventricular zone Present Regionally restricted 6d Expression was detected in BrdU-positive cells of the ventricular zone.

Specimen 6e: embryonic day 18.5 (more )
Note: Double labeled with BrdU (brown).
Structure Level Pattern Image Note
TS26: cerebral cortex ventricular zone Present Regionally restricted 6e Expression was detected in BrdU-positive cells of the ventricular zone.

Specimen 6f: embryonic day 18.5 (more )
Note: Double labeled with BrdU (brown).
Structure Level Pattern Image Note
TS26: cerebral cortex ventricular zone Present Regionally restricted 6f Expression was detected in BrdU-positive cells of the ventricular zone.

Specimen 7d: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebellum Present Regionally restricted 7d Expression was detected in the external germinal layer.

Specimen 7f: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebellum Present Regionally restricted 7f Expression was detected in the external germinal layer.

Specimen 10c: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: heart Weak 10c

Specimen 10d: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: heart Present 10d Maximum expression in the heart was seen at E18.5.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
04/16/2024
MGI 6.23
The Jackson Laboratory