Probe:	miR-20a probe
Probe preparation:	Antisense
Visualized with:	Fluorescein
Assay notes:	These data are summarized in Table 1.

Structure	Level	Pattern	Image	Note
TS5: inner cell mass	Weak		2A miR-20a
TS5: embryo	Weak	Regionally restricted	2A miR-20a	There is weak expression in the presumptive primitive endoderm.
TS5: trophectoderm	Moderate		2A miR-20a

Structure	Level	Pattern	Image	Note
TS7: epiblast	Absent		3B	There is no expression in the primitive ectoderm.
TS7: extraembryonic ectoderm	Weak		3B
TS7: primitive endoderm	Absent		3B	There is no expression in the visceral endoderm.

Structure	Level	Pattern	Image	Note
TS9: primitive streak	Absent		4C
TS9: embryo ectoderm	Moderate	Regionally restricted	4C	Expression is restricted to cells at least one cell layer removed from the posterior end of the primitive streak.
TS9: embryo mesoderm	Weak		4C
TS9: primitive endoderm	Weak	Regionally restricted	4C	There is weak expression in the visceral endoderm.