Probe:	E1 probe
Probe preparation:	Antisense labelled with digoxigenin
Visualized with:	Alkaline phosphatase
Assay notes:	Authors indicate that from E9.5-12.5 identical results were obtained for two Msx1 probes used in in situ hybridization assays. The two probes were E1 (MGI:4948291) and E2B (MGI:4948295).

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Present	Regionally restricted	3A	Expression was detected in the distal parts of the mandible primordium.

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch mandibular component	Present	Regionally restricted	3D	Expression was detected in the distal parts of the mandible primordium.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	3G	Expression was detected in the distal parts of the mandible.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mandibular component	Present	Regionally restricted	3J	Expression was detected in the distal parts of the mandible.

Structure	Level	Pattern	Image	Note
TS19: mandible primordium	Present	Graded	3M	Expression was detected in almost all the oral part of the mandible. An expression gradient was observed with levels higher in the distal region.

Structure	Level	Pattern	Image	Note
TS19: mandible primordium	Present	Graded	3P	xpression was detected in almost all the oral part of the mandible. An expression gradient was observed with levels higher in the distal region.

Structure	Level	Pattern	Image	Note
TS21: jaw epithelium	Present	Regionally restricted	5G	Expression was detected in the dental mesenchyme and in the dental epithelium.
TS21: jaw mesenchyme	Present	Regionally restricted	5G	Expression was detected in the dental mesenchyme and in the dental epithelium.

Structure	Level	Pattern	Image	Note
TS24: jaw epithelium	Present	Regionally restricted	5L	Expression was detected in the dental mesenchyme and in the dental epithelium.
TS24: jaw mesenchyme	Present	Regionally restricted	5L	Expression was detected in the dental mesenchyme and in the dental epithelium.

Structure	Level	Pattern	Image	Note
TS27: jaw	Present	Regionally restricted	5Q	Expression was detected in dental epithelium and mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: limb	Present	Widespread	6A	Expression was detected in the entire limb bud.

Structure	Level	Pattern	Image	Note
TS17: limb	Present	Regionally restricted	6D	Expression was detected in the area bordering the apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS19: limb	Present		6G