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Tg(Hsd17b1-icre/ERT2)3Casa
Transgene Detail
Summary
Symbol: Tg(Hsd17b1-icre/ERT2)3Casa
Name: transgene insertion 3, Emilio Casanova
MGI ID: MGI:4946612
Synonyms: Hsd17b1-iCreERT2
Transgene: Tg(Hsd17b1-icre/ERT2)3Casa  Location: unknown  
Alliance: Tg(Hsd17b1-icre/ERT2)3Casa page
Transgene
origin
Strain of Origin:  (C57BL/6 x CBA)F1
Transgene
description
Transgene Type:    Transgenic (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Tg(Hsd17b1-icre/ERT2)3Casa expression driven by 1 gene
 
Mutation detailsA 200kb BAC containing the Hsd17b1 gene was modified by homologous recombination to generate the construct. The open reading frame sequence for codon-improved cre recombinase (icre) fused to a mutated ligand-binding domain (LBD) of the human estrogen receptor (ERT2) was inserted into the exon containing the transcription initiation codon of the Hsd17b1 gene. The initial targeting vector also contained the bovine growth hormone polyA signal and an Frt-flanked ampicillin cassette. The amp marker was subsequently removed from targeted by clones upon electroporation of a plasmid expressing Flp recombinase. Purified BAC clones were injected into (C57BL/6 x CBA)F1 oocytes. Three lines expressing the transgene were generated. Lines 1 and 2 were shown to contain 2 copies of the insert by Southern blot, while line 3 had 8 copies. Line 3 mRNA levels of icre/ERT2 were highest in ovaries and this line was used for subsequent analyses. (J:170001)
Generation of Tg(Hsd17b1-icre/ERT2)3Casa
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Hsd17b1 (species not specified)
Summary of all recombinase alleles driven by Hsd17b1.
 

Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:170001 Grabner B, et al., A mouse tool for conditional mutagenesis in ovarian granulosa cells. Genesis. 2010 Oct 1;48(10):612-7
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory