Gene Expression Data
Assay Details
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Assay
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Reference:
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J:90402
Merrill BJ, et al., Tcf3: a transcriptional regulator of axis induction in the early embryo. Development. 2004 Jan;131(2):263-74
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4943200
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Gene symbol:
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Tcf7l1
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Gene name:
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transcription factor 7 like 1 (T cell specific, HMG box)
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Antibody:
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anti-Tcf3
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Detection system:
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Secondary antibody coupled to Texas Red
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Results
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Specimen
1F:
embryonic day 6.5
(more  )
Note: Double labeled: red - Tcf7l1; green - Ctnnb1 (assay MGI:4943195).
Specimen
1F:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Tcf7l1; green - Ctnnb1 (assay MGI:4943195).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS9: embryo ectoderm |
Present |
Widespread |
1F
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Expression in the nuclei is present throughout proximodistal length of the embryonic ectoderm before primitive streak induction.
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Specimen
1G:
embryonic day 7.5
(more )
Note: Double labeled: red - Tcf7l1; green - Cdh1 (assay MGI:4943198).
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Tcf7l1; green - Cdh1 (assay MGI:4943198).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo ectoderm |
Strong |
Regionally restricted |
1G
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Expression is intense in the ectoderm and mesoderm anterior to the node.
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| TS11: embryo mesoderm |
Strong |
Regionally restricted |
1G
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Expression is intense in the ectoderm and mesoderm anterior to the node.
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| TS11: primitive streak |
Ambiguous |
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1G
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Expression is either weak or absent.
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| TS11: embryo endoderm |
Present |
Regionally restricted |
1G
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Expression is present in a portion of the anterior endoderm.
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Specimen
1H:
embryonic day 7.5
(more )
Note: Double labeled: red - Tcf7l1; green - Ctnnb1 (assay MGI:4943195).
Specimen
1H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Tcf7l1; green - Ctnnb1 (assay MGI:4943195).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo ectoderm |
Present |
Regionally restricted |
1H
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Expression is diminished posteriorly during gastrulation.
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| TS11: embryo mesoderm |
Present |
Regionally restricted |
1H
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Expression is diminished posteriorly during gastrulation.
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Specimen
1I:
embryonic day 7.5
(more )
Note: Double labeled: red - Tcf7l1; green - Cdh1 (assay MGI:4943198).
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Tcf7l1; green - Cdh1 (assay MGI:4943198).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo |
Present |
Regionally restricted |
1I
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There is a gradient of expression, with the transition at or near the node. It rises from the posterior to the anterior, near the node.
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Analysis Tools