Nos1tm1.1(cre/ERT2)Zjh
Targeted Allele Detail
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Symbol: |
Nos1tm1.1(cre/ERT2)Zjh |
Name: |
nitric oxide synthase 1, neuronal; targeted mutation 1.1, Z Josh Huang |
MGI ID: |
MGI:4941223 |
Synonyms: |
nNOS-CreER-KI, nNOS-CreER-KI/KO, nNOS-CreERT2-KI, Nos1-CreER-KI, Nos1-CreERT2-KI |
Gene: |
Nos1 Location: Chr5:118004904-118096905 bp, + strand Genetic Position: Chr5, 57.29 cM, cytoband F
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Alliance: |
Nos1tm1.1(cre/ERT2)Zjh page
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Germline Transmission: |
Earliest citation of germline transmission:
J:151755
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Parent Cell Line: |
v6.5 (ES Cell)
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Strain of Origin: |
(C57BL/6 x 129S4/SvJae)F1
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Project Collection: |
Neuroscience Blueprint cre
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Nos1tm1.1(cre/ERT2)Zjh expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Nos1 (MGI:97360) |
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Mutation details: A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an frt-flanked neo cassette into the initiation codon of the neuronal nitric oxide synthase 1 locus (Nos1). This construct was electroporated into C57BL/6;129S-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S mice to originate the colony. Mutant mice were bred with Actin-FLPe mice to remove the neo selection cassette and the FLPe transgene was subsequently bred out of the line.
(J:151755)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Nos1
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009; |
All: |
36 reference(s) |
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