Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:115426
Zhuang X, et al., Targeted gene expression in dopamine and serotonin neurons of the mouse brain. J Neurosci Methods. 2005 Apr 15;143(1):27-32
|
Assay type:
|
In situ reporter (knock in)
|
MGI Accession ID:
|
MGI:4941154
|
Gene symbol:
|
Slc6a4
|
Gene name:
|
solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
|
Reporter:
|
Cre
|
Antibody:
|
anti-cre (Novagen/EMD)
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Results
|
Specimen
1d:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6 * SJL
|
Mutant Allele(s):
|
Slc6a4tm1(cre)Xz/Slc6a4+
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: raphe nucleus |
Strong |
|
1d
|
Staining is observed in serotonergic neurons in the Raphe nuclei.
|
Specimen
not shown:
(close )
Genetic Background:
|
involves: 129S1/Sv * C57BL/6 * SJL
|
Mutant Allele(s):
|
Slc6a4tm1(cre)Xz/Slc6a4+
|
Age:
|
postnatal
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|