Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito
Transgene Detail
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| Symbol: |
Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito |
| Name: |
transgene insertion 39-2, Kouichi Ito |
| MGI ID: |
MGI:4429540 |
| Synonyms: |
DR4, DR4-H2E, DRA/DRB1*0401-IE Tg, HLA-DR4, HLA-DR4-IE, HLA-DRA-IEalpha/HLA-DRBI*0401-IEbeta Tg, Tg(HLA-DRA/H2-Ea,HLA-DRB1*0401/H2-Eb)1Kito, Tg(HLA-DRA,HLA-DRB1)39-2Kito |
| Transgene: |
Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito Location: unknown
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| Alliance: |
Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito page
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| Transgene Type: |
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Transgenic (Humanized sequence, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Mutation details: A chimeric MHC class II complex was created by fusing the human peptide antigen binding domains of class II alpha and beta chains (HLA-DRA and HLA-DRBI*0401) to the remaining domains of murine class II alpha or beta chains (H2-Ea and H2-Eb1 genes that make up mouse I-Ed). The chimeric gene constructs were manipulated by replacing exon 2 of IEd-alpha or IEd-beta genomic DNA with gene fragments containing exon 2 of HLA-DRA or HLA-DRB1*0401 respectively. The native murine promoters were left intact. These transgene constructs were co-injected into blastocytes. Flow cytometry analysis indicates that B220+ cells co-express the chimeric MHC class II molecules. Line 39-2 is a representative of several lines generated. The chosen human alleles represent human serotype HLA-DR4.
(J:109908)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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| Original: |
J:109908 Ito K, et al., HLA-DR4-IE chimeric class II transgenic, murine class II-deficient mice are susceptible to experimental allergic encephalomyelitis. J Exp Med. 1996 Jun 1;183(6):2635-44 |
| All: |
25 reference(s) |
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Analysis Tools
