Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito
Name:	transgene insertion 39-2, Kouichi Ito
MGI ID:	MGI:4429540
Synonyms:	DR4, DR4-H2E, DRA/DRB1*0401-IE Tg, HLA-DR4, HLA-DR4-IE, HLA-DRA-IEalpha/HLA-DRBI*0401-IEbeta Tg, Tg(HLA-DRA/H2-Ea,HLA-DRB1*0401/H2-Eb)1Kito, Tg(HLA-DRA,HLA-DRB1)39-2Kito
Transgene:	Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito  Location: unknown
Alliance:	Tg(HLA-DRA,HLA-DRB1*0401)39-2Kito page

Strain of Origin:

C57BL/6

Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence) Mutation:    Insertion   Mutation details: A chimeric MHC class II complex was created by fusing the human peptide antigen binding domains of class II alpha and beta chains (HLA-DRA and HLA-DRBI*0401) to the remaining domains of murine class II alpha or beta chains (H2-Ea and H2-Eb1 genes that make up mouse I-Ed). The chimeric gene constructs were manipulated by replacing exon 2 of IEd-alpha or IEd-beta genomic DNA with gene fragments containing exon 2 of HLA-DRA or HLA-DRB1*0401 respectively. The native murine promoters were left intact. These transgene constructs were co-injected into blastocytes. Flow cytometry analysis indicates that B220+ cells co-express the chimeric MHC class II molecules. Line 39-2 is a representative of several lines generated. The chosen human alleles represent human serotype HLA-DR4. (J:109908)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available

Original:	J:109908 Ito K, et al., HLA-DR4-IE chimeric class II transgenic, murine class II-deficient mice are susceptible to experimental allergic encephalomyelitis. J Exp Med. 1996 Jun 1;183(6):2635-44
All:	23 reference(s)