Tg(Pgk1-lacZ)13Vmel
Transgene Detail
|
|
| Symbol: |
Tg(Pgk1-lacZ)13Vmel |
| Name: |
transgene insertion 13, Harald von Melchner |
| MGI ID: |
MGI:4888290 |
| Synonyms: |
loxP-lacZ, pgklxneolacZ, plnLacZ13, ppgklxneoLacZ |
| Transgene: |
Tg(Pgk1-lacZ)13Vmel Location: unknown
|
| Alliance: |
Tg(Pgk1-lacZ)13Vmel page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:47007
|
| Parent Cell Line: |
D3 (ES Cell)
|
| Strain of Origin: |
129S2/SvPas
|
|
| Transgene Type: |
|
Transgenic (Reporter) |
| Mutation: |
|
Insertion
|
| |
|
Mutation details: A construct was generated that consisted of two tandemly arrayed selectable marker genes expressed from the mouse Pgk1 promoter. The sequence coding for neomycin phosphotransferase flanked by loxP sites was 5' to the sequence encoding beta-galactosidase (lacZ; followed by the SV40 polyadenylation sequence). To prevent translation of lacZ from dicistronic transcripts, 2 copies of the bovine GH polyA sequence were inserted downstream of neo. Cre-mediated recombination will result in lacZ translation. The construct was electroporated into D3 ES cells. One clone (plnLacZ13) with one copy of the transgene was selected for further analysis and injected into C57BL/6 blastocysts.
(J:47007)
|
|
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
|
|
| Original: |
J:47007 Thorey IS, et al., Selective disruption of genes transiently induced in differentiating mouse embryonic stem cells by using gene trap mutagenesis and site-specific recombination. Mol Cell Biol. 1998 May;18(5):3081-8 |
| All: |
7 reference(s) |
|
Analysis Tools
