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Gene Expression Data
Assay Details
Assay
Reference: J:153498 Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
Assay type: RNA in situ
MGI Accession ID: MGI:4887884
Gene symbol: Lef1
Gene name: lymphoid enhancer binding factor 1
Probe: MH764 probe 635
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.
Results
Specimen 5A: embryonic day 14.5 (more )
Note: This data is from euxassay_018171.
Structure Level Pattern Image Note
TS23: collecting duct Strong euxassay_018171_12 Expression was detected in the ureteric tubules.
TS23: ureteric tip Absent euxassay_018171_12
TS23: cap mesenchyme Absent euxassay_018171_12 Expression was not detected in the condensing mesenchyme.
TS23: renal vesicle Absent euxassay_018171_12 Expression was not detected in the renal vesicles/comma-shaped bodies.
TS23: s-shaped body Absent euxassay_018171_12
TS23: cortical renal tubule Strong euxassay_018171_12 Expression was detected in the proximal tubules.
TS23: maturing glomerular tuft Absent euxassay_018171_12 Expression was not detected in the glomeruli.
TS23: renal vasculature Strong euxassay_018171_12 Expression was detected in the capillaries.
TS23: renal cortical interstitium Strong euxassay_018171_12 Expression was detected in the stroma.
TS23: renal capsule Absent euxassay_018171_12
Specimen Table S8: embryonic day 14.5 (more )
Note: This data is from euxassay_018171.
Structure Level Pattern Image Note
TS23: neocortex Weak euxassay_018171_12
TS23: hippocampus Moderate euxassay_018171_12
TS23: corpus striatum Absent euxassay_018171_12
TS23: telencephalon septum Absent euxassay_018171_12
TS23: thalamus Moderate euxassay_018171_12
TS23: hypothalamus Strong euxassay_018171_12
TS23: pretectum Moderate euxassay_018171_12
TS23: superior colliculus Strong euxassay_018171_12
TS23: inferior colliculus Strong euxassay_018171_12
TS23: midbrain tegmentum Weak euxassay_018171_12
TS23: cerebellum Strong euxassay_018171_12
TS23: spinal cord Absent euxassay_018171_12
TS23: skin Strong euxassay_018171_12
TS23: lung Moderate euxassay_018171_12
TS23: metanephros Strong euxassay_018171_12
TS23: liver Moderate euxassay_018171_12
TS23: thyroid gland Absent euxassay_018171_12
TS23: pituitary gland Weak euxassay_018171_12
TS23: pancreas Absent euxassay_018171_12
TS23: adrenal gland Absent euxassay_018171_12
TS23: heart Strong euxassay_018171_12
TS23: thymus primordium Strong euxassay_018171_12
TS23: vertebral axis musculature Absent euxassay_018171_12
TS23: axial skeleton Moderate euxassay_018171_12
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory