Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(MMTV-tTA,PyVT-tetO-cre)6Atai
Name:	transgene insertion 6, Atsu Aiba
MGI ID:	MGI:4881748
Synonyms:	MMTV-LTR-tTA/mT-TRE-cre Tg
Transgene:	Tg(MMTV-tTA,PyVT-tetO-cre)6Atai  Location: unknown
Alliance:	Tg(MMTV-tTA,PyVT-tetO-cre)6Atai page

Strain of Origin:

C57BL/6N

Transgene Type:    Transgenic (Inducible, Recombinase, Transactivator) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Tg(MMTV-tTA,PyVT-tetO-cre)6Atai expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note virus MMTV   Mutation details: One transgenic vector consisted of cDNA encoding the tetracycline transactivator tTA (containing the tet repressor, TetR and the transcriptional activation domain of protein 16 of the herpes simplex virus, VP16) downstream of the MMTV-LTR promoter. The tTA fragment is flanked by the acceptor splice site and the polyadenylation site from the rabbit beta-globin gene. A second transgene was composed of the polyomavirus middle t antigen (mT; PyVT) cDNA upstream of a bidirectional tetracycline responsive element (with heptamerized tet operator sequences - TetR binding sites flanked by CMV promoters). The cre sequence was inserted after tetO between an acceptor splice site and polyadenylation site. In this construct, simulataneous expression of both cre and PyVT is inducible. The 2 purified constructs were coinjected into fertilized C57BL/6N oocytes. Four founders were generated with line 6 transmitting the transgenes over many generations. (J:168057)

Activity:	Tissue activity of this recombinase allele
Driver:	MMTV (virus) Summary of all recombinase alleles driven by MMTV.

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:168057 Sakahara M, et al., The simultaneous induction of tumorigenesis and Cre-loxP recombination in mice. Kobe J Med Sci. 2009;54(6):E279-89
All:	1 reference(s)