Csf2ra RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:152946 Sferruzzi-Perri AN, et al., Csf2 null mutation alters placental gene expression and trophoblast glycogen cell and giant cell abundance in mice. Biol Reprod. 2009 Jul;81(1):207-21
Assay type:	RT-PCR
MGI Accession ID:	MGI:4881673
Gene symbol:	Csf2ra
Gene name:	colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)

Probe:	Csf2ra-pA, Csf2ra-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Dissected labyrinthine zone and junctional zone tissue was used for analysis.

Results

Image: 5B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
LZ WT	E14.5 (a)	TS22: labyrinthine zone TS28: placenta	Present	2 µg; total RNA	C57BL/6		Not Specified
JZ WT	E14.5 (a)	TS22: spongiotrophoblast TS22: trophoblast giant cell TS28: placenta	Present (b)	2 µg; total RNA	C57BL/6		Not Specified
LZ KO	E14.5 (a)	TS22: labyrinthine zone TS28: placenta	Present	2 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6	Csf2^tm1Ard/Csf2^tm1Ard	Not Specified
JZ KO	E14.5 (a)	TS22: spongiotrophoblast TS22: trophoblast giant cell TS28: placenta	Present (c)	2 µg; total RNA	involves: 129P2/OlaHsd * C57BL/6	Csf2^tm1Ard/Csf2^tm1Ard	Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) Expression was 6.5-fold greater in the junctional zone compared with the labyrinthine zone in wild-type.
(c) Expression was 11.4-fold greater in the junctional zone compared with the labyrinthine zone in mutants. There was a 1.8-fold increase in expression in junctional zone tissue compared with wild-type tissue.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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