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Gene Expression Data
Assay Details
Assay
Reference: J:145843 Duncan MK, et al., Prox1 is differentially localized during lens development. Mech Dev. 2002 Mar;112(1-2):195-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:4867410
Gene symbol: Prox1
Gene name: prospero homeobox 1
Antibody: anti-Prox1
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1A,B: embryonic day 9.5 (more )
Note: (B) Higher magnification of the lens placode and optic vesicle shown in A. Double labeled: red - Prox1; green - DNA.
Structure Level Pattern Image Note
TS15: lens placode Present 1B , 1A Expression is in the cytoplasm.
TS15: surface ectoderm Present Regionally restricted 1A , 1B Expression is in the head ectoderm adjacent to the lens placode.
Specimen 1C,D: embryonic day 10.5 (more )
Note: (D) Higher magnification of the tip of the closing lens pit boxed in panel B. Double labeled: red - Prox1; green - DNA.
Structure Level Pattern Image Note
TS17: lens pit Present Regionally restricted 1D , 1C Protein is primarily nuclear in the posterior portion of the lens pit.
TS17: eye surface ectoderm Weak Regionally restricted 1C , 1D Expression is in the head ectoderm that will give rise to the corneal epithelium.
Specimen 1 E,F,G,H: embryonic day 10.5 (more )
Note: Late 10.5 dpc embryo. (F) Higher magnification of the area boxed in white in panel E. Double labeled: red - Prox1; green - DNA. (G) Same image shown in F with the DNA signal subtracted. (H) Higher magnification of the area boxed in yellow in panel E.
Structure Level Pattern Image Note
TS17: lens pit Present Regionally restricted 1H , 1G , 1F , 1E Protein is localized to the posterior portion of the lens vesicle.
Specimen 1I: embryonic day 12.5 (more )
Note: Double labeled: red - Prox1; green - DNA.
Structure Level Pattern Image Note
TS20: lens vesicle Present Regionally restricted 1I Protein is detected in the nuclei of primary lens fibers.
Specimen 1J,K, L: embryonic day 16.5 (more )
Note: Double labeled: red - Prox1; green - DNA. (K) Same image shown in (J) with the DNA signal subtracted. (L) Higher magnification of the area boxed in panel (J).
Structure Level Pattern Image Note
TS24: lens fiber Strong Regionally restricted 1K , 1L , 1J Expression is in lens fibers at the transition zone.
TS24: lens epithelium Present 1K , 1L , 1J
Specimen Not shown, E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: lens vesicle Present Regionally restricted Protein is localized to the posterior portion of the lens vesicle.
Specimen Not shown, Adult: postnatal adult (more )
Structure Level Pattern Image Note
TS28: lens fiber Present In nuclear regions.
TS28: lens epithelium Present Mostly cytoplasmic.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
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