Myo3a RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:151359 Lelli A, et al., Tonotopic gradient in the developmental acquisition of sensory transduction in outer hair cells of the mouse cochlea. J Neurophysiol. 2009 Jun;101(6):2961-73
Assay type:	RT-PCR
MGI Accession ID:	MGI:4867257
Gene symbol:	Myo3a
Gene name:	myosin IIIA

Probe:	Myo3a-pA, Myo3a-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. The data are shown as a 3D plot with relative measurements in 4 separate areas of the organ of Corti (basal end, mid-basal, mid-apical, and apical end).

Results

Image: 7C

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	122 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
E17	E17.0 (a)	TS25: cochlea	Present (b)	0.1 µg; total RNA	Swiss Webster		Not Specified
E18	E18.0 (a)	TS26: cochlea	Present	0.1 µg; total RNA	Swiss Webster		Not Specified
P0	P0	TS27: cochlea	Present	0.1 µg; total RNA	Swiss Webster		Not Specified
P2	P2	TS27: cochlea	Present (c)	0.1 µg; total RNA	Swiss Webster		Not Specified
P4	P4	TS28: cochlea	Present	0.1 µg; total RNA	Swiss Webster		Not Specified
P6	P6	TS28: cochlea	Present	0.1 µg; total RNA	Swiss Webster		Not Specified
P8	P8	TS28: cochlea	Present (d)	0.1 µg; total RNA	Swiss Webster		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) There is a gradient of expression with the lowest levels in the apex and progressively higher expression levels toward the base.
(c) There is a rise in expression from EI7 to PO-P2 in the apical half such that by P2 levels are more uniform across the entire cochlea.
(d) Higher expression in the apex than in the base.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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