Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:163866
Goto M, et al., Speriolin is a novel human and mouse sperm centrosome protein. Hum Reprod. 2010 Aug;25(8):1884-94
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:4850009
|
Gene symbol:
|
Spatc1
|
Gene name:
|
spermatogenesis and centriole associated 1
|
Antibody:
|
speriolin antibody
|
Detection system:
|
Secondary antibody coupled to colloidal gold
|
Assay notes:
|
Electron microscopic immunolocalization. Fixative was 4% paraformaldehyde and 0.5% glutaraldehyde, and post-fixative was 1% osmium tetroxide. Samples were embedded in LR White resin.
|
|
Results
|
Specimen
3A:
postnatal adult
(more )
Note: longitudinal section
Specimen
3A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Resin/plastic
|
Note:
|
longitudinal section
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: germ cell of testis |
Present |
Regionally restricted |
3A
|
Immunoreactivity was detected specifically at the periphery of the columns of the connecting piece of the neck region.
|
Specimen
3B:
postnatal adult
(more )
Note: transverse section
Specimen
3B:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
|
Sex:
|
Male
|
Type:
|
whole mount
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Resin/plastic
|
Note:
|
transverse section
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: germ cell of testis |
Present |
Regionally restricted |
3B
|
Immunoreactivity was detected specifically at the periphery of the columns of the connecting piece of the neck region.
|
|