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Gene Expression Data
Assay Details
Assay
Reference: J:138858 Georgas K, et al., Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney. Histochem Cell Biol. 2008 Nov;130(5):927-42
Assay type: Immunohistochemistry
MGI Accession ID: MGI:4849230
Gene symbol: Wt1
Gene name: WT1 transcription factor
Antibody: 6F-H2 (M3561)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes: The Fig. 5 specimens are also entries in the GUDMAP database (GUDMAP:8200).
Results
Specimen 2: postnatal adult (more )
Structure Level Pattern Image Note
TS28: visceral epithelium of mature renal corpuscle Present 2A Wt1
Specimen 5a: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241).
Structure Level Pattern Image Note
TS23: cap mesenchyme Moderate 5a , 5b
TS23: nephrogenic interstitium Moderate 5a , 5b
TS23: early nephron Present 5a , 5b Expression is present in early nephrons.
TS23: visceral epithelium of maturing renal corpuscle Present 5a , 5b Expression is present in the visceral epithelium of mature nephrons.
TS23: s-shaped body Present 5b , 5a
TS23: renal cortical interstitium Moderate Single cells 5a , 5c Expression is present in cells immediately surrounding the cortical and medullary collecting ducts.
TS23: renal medullary interstitium Moderate Single cells 5a , 5c Expression is present in cells immediately surrounding the cortical and medullary collecting ducts.
TS23: renal capsule Moderate 5c , 5a
Specimen 5d: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241).
Structure Level Pattern Image Note
TS23: pretubular aggregate Moderate 5d
TS23: renal vesicle Moderate Regionally restricted 5d Expression is present in the proximal renal vesicle.
Specimen 5e: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241).
Structure Level Pattern Image Note
TS23: pretubular aggregate Moderate 5e
TS23: renal vesicle Moderate Regionally restricted 5e Expression is present in the proximal renal vesicle.
Specimen 5f: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241).
Structure Level Pattern Image Note
TS23: lower limb of comma-shaped body Strong 5f Expression is present in the elongated cells of the lower limb of the comma-shaped body.
Specimen 5g: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241). This is an early S-shaped body.
Structure Level Pattern Image Note
TS23: parietal epithelium of s-shaped body Strong 5g Expression is present in the visceral and parietal epithelium of the proximal segment of the S-shaped body.
TS23: visceral epithelium of s-shaped body Strong 5g Expression is present in the visceral and parietal epithelium of the proximal segment of the S-shaped body.
Specimen 5h: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241). This is an S-shaped body.
Structure Level Pattern Image Note
TS23: parietal epithelium of s-shaped body Strong 5h Expression is present in the visceral and parietal epithelium of the proximal segment of the S-shaped body.
TS23: visceral epithelium of s-shaped body Strong 5h Expression is present in the visceral and parietal epithelium of the proximal segment of the S-shaped body.
Specimen 5i: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241). This is a late S-shaped body.
Structure Level Pattern Image Note
TS23: parietal epithelium of s-shaped body Strong 5i Expression is present in the visceral and parietal epithelium of the proximal segment of the S-shaped body.
TS23: visceral epithelium of s-shaped body Strong 5i Expression is present in the visceral and parietal epithelium of the proximal segment of the S-shaped body.
Specimen 5j: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241). This is a capillary loop nephron.
Structure Level Pattern Image Note
TS23: parietal epithelium of capillary loop renal corpuscle Strong 5j Expression is present in the visceral and parietal epithelium of the renal corpuscle of the capillary loop.
TS23: visceral epithelium of capillary loop renal corpuscle Strong 5j Expression is present in the visceral and parietal epithelium of the renal corpuscle of the capillary loop.
Specimen 5k: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241). This is a capillary loop nephron.
Structure Level Pattern Image Note
TS23: parietal epithelium of capillary loop renal corpuscle Strong 5k Expression is present in the visceral and parietal epithelium of the renal corpuscle of the capillary loop.
TS23: visceral epithelium of capillary loop renal corpuscle Strong 5k Expression is present in the visceral and parietal epithelium of the renal corpuscle of the capillary loop.
Specimen 5l: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241). This is a maturing nephron.
Structure Level Pattern Image Note
TS23: visceral epithelium of maturing renal corpuscle Strong 5l
TS23: parietal epithelium of maturing renal corpuscle Weak 5l Expression is present in the parietal epithelium of the renal corpuscles of maturing nephrons.
Specimen 5m: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Wnt4 (assay MGI:4849241). This is a maturing nephron.
Structure Level Pattern Image Note
TS23: visceral epithelium of maturing renal corpuscle Strong 5m
Specimen 6B: embryonic day 15.5 (more )
Note: This schematic summarizes the stage I-II nephron gene expression data.
Structure Level Pattern Image Note
TS23: renal vesicle Present Regionally restricted 6B Expression is present in the proximal renal vesicle.
TS23: pretubular aggregate Present 6B
TS23: cap mesenchyme Present 6B
TS23: nephrogenic interstitium Present 6B Expression is present in the nephrogenic zone interstititium (uninduced mesenchyme).
TS23: renal capsule Present 6B
TS23: s-shaped body Present Regionally restricted 6B Expression is present in the proximal portion of the developing nephron.
TS23: visceral epithelium of s-shaped body Present 6B
TS23: parietal epithelium of s-shaped body Present 6B
TS23: upper limb of comma-shaped body Present 6B
Specimen 6C: embryonic day 15.5 (more )
Note: This schematic summarizes the stage III-IV nephron gene expression data.
Structure Level Pattern Image Note
TS23: visceral epithelium of capillary loop renal corpuscle Present 6C
TS23: parietal epithelium of capillary loop renal corpuscle Present 6C
TS23: visceral epithelium of maturing renal corpuscle Present 6C
Specimen 7Db: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Jag1 (assay MGI:4849248).
Structure Level Pattern Image Note
TS23: proximal segment of s-shaped body Present 7Db
Specimen 7Dd: embryonic day 15.5 (more )
Note: Double labeled: orange - Wt1; blue - Jag1 (assay MGI:4849248).
Structure Level Pattern Image Note
TS23: visceral epithelium of capillary loop renal corpuscle Present 7Dd
TS23: visceral epithelium of maturing renal corpuscle Present 7Dd
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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