Summary | Transgene origin | Transgene description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(Thy1-Clomeleon)13Gjau
Name:	transgene insertion 13, George Augustine
MGI ID:	MGI:4847926
Synonyms:	Tg(Thy1-CFP,Topaz)CLM13Gjau, Tg(Thy1-CFP,YFP)CLM13Gjau
Transgene:	Tg(Thy1-Clomeleon)13Gjau  Location: unknown
Alliance:	Tg(Thy1-Clomeleon)13Gjau page

Strain of Origin:

ICR

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: A transgenic construct containing Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2) was injected into fertilized ICR mouse eggs. Founder line CLM13 was subsequently established. Transgenic mice express Clomeleon, a fluorescent fusion protein containing CFP and topaz YFP that acts as a ratiometric indicator for chloride ions, under the control of the mouse Thy1, thymus cell antigen 1, theta, promoter (Thy1.2). Transgene expression in founder line CLM13 is detected at high levels in the hippocampal dentate gyrus and CA1 region, amygdala, and nuclei and mossy fibers of the cerebellum. No expression is detected in the external plexi form layer, mitral cells or internal plexiform layer of the olfactory bulb, layer 1 of the neocortex, cerebellar granule or purkinje cells, or retinal biopolar and amacrine cells. In the presence of low chloride ion concentrations, YFP fluoresces as a FRET (Fluorescence Resonance Energy Transfer ) acceptor for CFP. As the chloride ion concentration increases, YFP fluorescence decreases and CFP fluorescence increases. Using an appropriate calibration curve, the ratio of YFP and CFP fluorescence peaks indicate [Cl-]. (J:166512)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

1 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:165251 Duebel J, et al., Two-photon imaging reveals somatodendritic chloride gradient in retinal ON-type bipolar cells expressing the biosensor Clomeleon. Neuron. 2006 Jan 5;49(1):81-94
All:	4 reference(s)