Gene Expression Data
Assay Details
Assay
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Reference:
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J:71338
Chung WW, et al., Developmental expression of neural cell adhesion molecules in the mouse neocortex and olfactory bulb. J Comp Neurol. 1991 Dec 8;314(2):290-305
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4844167
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Gene symbol:
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Ncam1
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Gene name:
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neural cell adhesion molecule 1
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Antibody:
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12F8 antibody
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
5A:
embryonic day 12.5
(more )
Specimen
5A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
5D:
embryonic day 14.5
(more )
Specimen
5D:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 14.5
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Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cerebral cortex |
Present |
Regionally restricted |
5D
|
Authors report patterns of expression similar to that seen with polyclonal N-CAM antibody.
|
Specimen
5G:
embryonic day 16.5
(more )
Specimen
5G:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cerebral cortex |
Present |
Regionally restricted |
5G
|
Authors report patterns of expression similar to that seen with polyclonal N-CAM antibody.
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Specimen
5J:
embryonic day 18.5
(more )
Specimen
5J:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex |
Present |
Regionally restricted |
5J
|
Authors report patterns of expression similar to that seen with polyclonal N-CAM antibody.
|
Specimen
7A:
postnatal day 0
(more )
Specimen
7A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 0
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cerebral cortex |
Moderate |
Regionally restricted |
7A
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Authors report patterns of expression similar to that seen with polyclonal N-CAM antibody.
|
Specimen
not shown:
postnatal day 6
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal day 6
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cerebral cortex |
Present |
Regionally restricted |
|
Expression in cortex starts to wane, except in layer 1 and white matter.
|
Specimen
7D:
postnatal day 14
(more )
Specimen
7D:
(close )
Genetic Background:
|
CD-1
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Age:
|
postnatal day 14
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
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Specimen
7G:
postnatal adult
(more )
Specimen
7G:
(close )
Genetic Background:
|
CD-1
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Age:
|
postnatal adult
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: neocortex |
Trace |
Widespread |
7G
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Barely detectable expression throughout whole depth of neocortex.
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Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
10A:
embryonic day 16.5
(more )
Specimen
10A:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 16.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
10D:
postnatal day 0
(more )
Specimen
10D:
(close )
Genetic Background:
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CD-1
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Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
10G:
postnatal day 14
(more )
Specimen
10G:
(close )
Genetic Background:
|
CD-1
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Age:
|
postnatal day 14
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
10J:
postnatal adult
(more )
Specimen
10J:
(close )
Genetic Background:
|
CD-1
|
Age:
|
postnatal adult
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
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