Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:92504
Harfe BD, et al., Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell. 2004 Aug 20;118(4):517-28
|
|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:4838650
|
|
Gene symbol:
|
Shh
|
|
Gene name:
|
sonic hedgehog
|
|
Reporter:
|
GFP
|
|
|
Direct Detection
|
|
|
Results
|
Specimen
1B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Shhtm1(EGFP/cre)Cjt/Shh+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
1B
|
Expression is observed in the posterior border of the distal limb bud.
|
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Mutant Allele(s):
|
Shhtm1(EGFP/cre)Cjt/Shh+
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
Not Specified
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
Regionally restricted |
|
Outside the limb expression is observed in all the tissues where endogeneous expression has previously been reported.
|
|