Probe:	Tes-56
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex mantle layer	Present	Regionally restricted	3f	Expression in the mantle zone of the cerebral cortex in postmitotic cells.
TS20: cerebral cortex intermediate zone	Present		3f
TS20: cortical plate	Present		3f
TS20: telencephalon ventricular layer	Absent		3f	No expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4e	Expression in primordia of paleocortex, neocortex and archicortex. Expression in primordia of anterior olfactory nucleus, diagonal band nucleus, eminentia thalami, and bed nucleus of stria medullaris.
TS20: lateral ganglionic eminence	Absent		4e
TS20: thalamus	Absent		4e	No expression in dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4f	Expression in primordia of paleocortex, neocortex and archicortex. Expression in primordia of anterior olfactory nucleus and diagonal band nucleus.
TS20: lateral ganglionic eminence	Absent		4f
TS20: choroid invagination	Absent		4f

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4g	Expression in primordia of paleocortex, neocortex and archicortex. Expression in the eminentia thalami.
TS20: lateral ganglionic eminence	Absent		4g
TS20: thalamus	Absent		4g	No expression in dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	4h	Expression in primordia of paleocortex, neocortex and archicortex. Expression in the bed nucleus of stria medullaris.
TS20: caudal ganglionic eminence	Present		4h

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	5c	Expression rostral to a boundary in the superficial zone of the neocortical plate, which shifts caudally in more medial regions of the neocortex.
TS24: cortical plate	Present	Regionally restricted	5c	Expression in the superficial cortical plate is 6-fold greater rostrally than caudally. Expression in deep layers was not significantly different.
TS24: skin	Present		5c

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	5d	Expression rostral to a boundary in the superficial zone of the neocortical plate, which shifts caudally in more medial regions of the neocortex.
TS24: skin	Present		5d

Structure	Level	Pattern	Image	Note
TS24: thalamus	Absent			No expression in dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Regionally restricted	6e	Expression rostral to a boundary in the superficial zone of the neocortical plate.
TS26: amygdala	Present		6e	Expression in parts of the the amygdala.
TS26: hippocampus	Present	Regionally restricted	6e	Discontinuity of expression which approximates boundary of hippocampus with subiculum.
TS26: skin	Present		6e

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Regionally restricted	6f	Expression rostral to a boundary in the superficial zone of the neocortical plate.
TS26: hippocampus	Present	Regionally restricted	6f	Discontinuity of expression which approximates boundary of hippocampus with subiculum.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Regionally restricted	6g	Expression rostral to a boundary in the superficial zone of the neocortical plate.
TS26: cortical subplate	Present		6g	Expression in a thin layer of cells between the cortical plate and the intermediate zone which may be the subplate.
TS26: amygdala	Present	Regionally restricted	6g	Expression in parts of the the amygdala.
TS26: hippocampus	Present	Regionally restricted	6g	Discontinuity of expression which approximates boundary of hippocampus with subiculum. Expression in subiculum decreases abruptly at the hippocampal primordium.
TS26: thalamus	Absent		6g	No expression in dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS26: cortical plate	Present	Regionally restricted	6h	Expression rostral to a boundary in the superficial zone of the neocortical plate.
TS26: amygdala	Present	Regionally restricted	6h	Expression in parts of the the amygdala.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present			Expression rostral to a boundary in the superficial zone of the neocortical plate.

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb	Present	Regionally restricted	7a	Expression in the olfactory bulb, largely in a single layer that appears to correspond to mitral cells.
TS27: main olfactory bulb mitral cell layer	Present		7a	Expression in the olfactory bulb, largely in a single layer that appears to correspond to mitral cells.
TS27: nucleus of diagonal band	Present	Regionally restricted	7a	Expression in the vertical limb of the diagonal band.
TS27: deep cerebellar nucleus	Present		7a

Structure	Level	Pattern	Image	Note
TS28: anterior olfactory nucleus	Present		7b
TS28: telencephalon	Present	Regionally restricted	7b	Expression in a thin strip of cells at the interface of white matter and neocortex; may correspond to the subplate.
TS28: neocortex	Present		7b
TS28: cerebral cortex	Present	Regionally restricted	7b	Much less expression in a zone superficial to layer 6 that may correspond to a sublamina of layer 5.
TS28: cortical layer VI	Present		7b
TS28: cortical layer V	Present	Regionally restricted	7b	Expression in scattered cells. Thin line of expressing cells becomes much wider.
TS28: hippocampus	Present	Regionally restricted	7b	Attenuation of expression at the boundary of the subiculum with the hippocampus CA1.
TS28: nucleus of diagonal band	Present	Regionally restricted	7b	Expression in the horizontal limb of the diagonal band.
TS28: deep cerebellar nucleus	Present		7b
TS28: dorsal thalamus	Present		7b

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Regionally restricted	7c
TS28: cortical layer V	Present	Regionally restricted	7c	Expression in scattered cells. Thin line of expressing cells becomes much wider.
TS28: accessory olfactory bulb	Present		7c
TS28: anterior olfactory nucleus	Present		7c
TS28: entorhinal cortex	Present		7c
TS28: nucleus of diagonal band	Present	Regionally restricted	7c	Expression in the vertical limb of the diagonal band.
TS28: hippocampus	Present	Regionally restricted	7c	Attenuation of expression at the boundary of the subiculum with the hippocampus CA1.
TS28: dentate gyrus	Strong		7c	High levels of expression in hippocampus beginning at approximately CA3 and continuing into the dentate gyrus.
TS28: hippocampus CA3	Strong		7c	High levels of expression in hippocampus beginning at approximately CA3 and continuing into the dentate gyrus.
TS28: thalamus	Present	Regionally restricted	7c	Expression nucleus of the stria medullaris.
TS28: dorsal thalamus	Present		7c

Structure	Level	Pattern	Image	Note
TS28: thalamus	Weak

Structure	Level	Pattern	Image	Note
TS28: endopiriform nucleus	Present		7d
TS28: piriform cortex	Present		7d
TS28: telencephalon	Present	Regionally restricted	7d	Thin strip of expressing cells between white matter and layer 6 that may correspond to the subplate.
TS28: neocortex	Present	Regionally restricted	7d
TS28: cortical layer VI	Present		7d
TS28: cortical layer IV	Weak		7d
TS28: cortical layer V	Weak		7d
TS28: cortical layer I	Strong		7d
TS28: cortical layer II	Strong		7d
TS28: cortical layer III	Strong		7d
TS28: claustrum	Present		7d
TS28: entorhinal cortex	Present		7d
TS28: hippocampus	Present		7d
TS28: nucleus of lateral olfactory tract	Present		7d
TS28: amygdala	Present	Regionally restricted	7d	Expression in parts of the amygdala.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted	7d	Expression in Purkinge cells in the lateral cerebellum.

Structure	Level	Pattern	Image	Note
TS28: thalamus	Weak

Structure	Level	Pattern	Image	Note
TS28: indusium griseum	Present
TS28: subiculum	Present
TS28: taenia tecta	Present
TS28: locus coeruleus	Present	Regionally restricted		Nest of expressing cells in the region of the locus coeruleus.