Gene Expression Data
Assay Details
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Assay
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Reference:
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J:137506
Yoshikawa F, et al., Opalin, a transmembrane sialylglycoprotein located in the central nervous system myelin paranodal loop membrane. J Biol Chem. 2008 Jul 25;283(30):20830-40
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4837137
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Gene symbol:
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Opalin
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Gene name:
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oligodendrocytic myelin paranodal and inner loop protein
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Antibody:
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anti-Opalin
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Detection system:
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Secondary antibody coupled to colloidal gold
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Results
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Specimen
7A:
postnatal week 2
(more  )
Specimen
7A:
(close  )
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Genetic Background:
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ICR
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Age:
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postnatal week 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: optic nerve |
Present |
Regionally restricted |
7A
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Expression is localized around the inner loop (or adaxonal) membrane (or inner mesaxon). It is not seen in the compact myelin lamellae, the outer loop (abaxonal) membrane or the axo-glial junctions.
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Specimen
7B:
postnatal week 2
(more )
Specimen
7B:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal week 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: optic nerve |
Present |
Regionally restricted |
7B
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Immunogold particles are localized on the cytoplasmic side immediately below the paranodal loop membranes. Pairs of immunogold particles are often observed on apposing membranes. No immunogold was detected in the axo-glial junctions of paranodes.
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Specimen
7C:
postnatal week 2
(more )
Specimen
7C:
(close )
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Genetic Background:
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ICR
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Age:
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postnatal week 2
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: optic nerve |
Present |
Regionally restricted |
7C
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Immunogold particles are localized on the cytoplasmic side immediately below the paranodal loop membranes. Pairs of immunogold particles are often observed on apposing membranes. No immunogold was detected in the axo-glial junctions of paranodes.
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Analysis Tools