Gene Expression Data
Assay Details
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Assay
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Reference:
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J:163441
Yang DH, et al., Wnt5a is required for endothelial differentiation of embryonic stem cells and vascularization via pathways involving both Wnt/beta-catenin and protein kinase Calpha. Circ Res. 2009 Feb 13;104(3):372-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4830879
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Gene symbol:
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Pecam1
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Gene name:
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platelet/endothelial cell adhesion molecule 1
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Antibody:
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anti-PECAM-1
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
7B:
embryonic day 14.5; Wnt5atm1Amc/Wnt5a +
(more  )
Specimen
7B:
(close  )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5a+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
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7B +/-
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There is staining in what are presumed to be endothelial cells in the marginal layer of the spinal cord.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Absent |
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7B -/-
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There is no staining of presumed endothelial cells in the marginal layer of the spinal cord.
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Specimen
7C sagittal:
embryonic day 14.5; Wnt5atm1Amc/Wnt5a +
(more )
Specimen
7C sagittal:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5a+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
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7C +/- upper
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There is staining in what are presumed to be endothelial cells in the marginal layer of the spinal cord.
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Specimen
7C sagittal:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Absent |
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7C -/- upper
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There is no staining of presumed endothelial cells in the marginal layer of the spinal cord.
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Specimen
7C transverse:
embryonic day 14.5; Wnt5atm1Amc/Wnt5a +
(more )
Specimen
7C transverse:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5a+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Present |
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7C +/- lower
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There is staining in what are presumed to be endothelial cells in the marginal layer of the spinal cord and in the dorsal root gangia.
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Specimen
7C transverse:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd
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Mutant Allele(s):
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Wnt5atm1Amc/Wnt5atm1Amc
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: blood vessel |
Absent |
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7C -/- lower
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There is no staining of presumed endothelial cells in the marginal layer of the spinal cord nor in the dorsal root ganglia.
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Analysis Tools
