Tg(Tph2-cre/ERT2)1.38Zi
Transgene Detail
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| Symbol: |
Tg(Tph2-cre/ERT2)1.38Zi |
| Name: |
transgene insertion 1.38, Dusan Bartsch |
| MGI ID: |
MGI:4830865 |
| Synonyms: |
1.38, TPH2-CreERT2 |
| Transgene: |
Tg(Tph2-cre/ERT2)1.38Zi Location: unknown
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| Alliance: |
Tg(Tph2-cre/ERT2)1.38Zi page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Tph2-cre/ERT2)1.38Zi expression driven by
1 gene
Transgene expression driven by:
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Mutation details: A 177 kb PAC (P1-derived artificial chromosome; pPAC4) containing the full-length murine Tph2 gene and flanking sequences were modified by recombineering methods in EL250 bacteria prior to use for transgene construction. First, a chloramphenicol resistance gene replaced the kanamycin gene in the PAC backbone. Then a cassette encoding cre fused to a mutated human estrogen receptor (ER) ligand-binding domain (LBD; creERT2) and an FRT-flanked kanamycin resistance gene was integrated into the ATG start codon. A 23 bp sequence downstream of the ATG codon was deleted as it contained other in-frame ATG start sites in exon 1 of Tph2. Arabinose-induced FLP expression deleted the kanamycin cassette. The modified Tph2 sequence was excised from the PAC backbone and purified for injection into C57BL/6N oocytes. Five founder lines were obtained (1.21, 1.23, 1.27, 1.35, and 1.38). Lines 1.21 and 1.38 produced the highest recombination efficiency in serotonergic neurons with tamoxifen induction (91 and 90% respectively). Line 1.38 was investigated most extensively.
(J:164111)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Tph2
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:164111 Weber T, et al., Inducible gene manipulations in serotonergic neurons. Front Mol Neurosci. 2009;2:24 |
| All: |
6 reference(s) |
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Analysis Tools
