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Gene Expression Data
Assay Details
Assay
Reference: J:153498 Diez-Roux G, et al., A high-resolution anatomical atlas of the transcriptome in the mouse embryo. PLoS Biol. 2011;9(1):e1000582
Assay type: RNA in situ
MGI Accession ID: MGI:4826660
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Probe: T36676
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramide-biotin/streptavidin amplification step was included in the in situ hybridization procedure.
Results
Specimen euxassay_009463_01: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: neural retina Strong Regionally restricted euxassay_009463_01
Specimen euxassay_009463_02: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: neural retina Strong Regionally restricted euxassay_009463_02
Specimen euxassay_009463_03: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_03
TS23: neural retina Strong Regionally restricted euxassay_009463_03
Specimen euxassay_009463_04: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_04
TS23: facial ganglion Strong Regionally restricted euxassay_009463_04
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_04
Specimen euxassay_009463_05: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_05
TS23: facial ganglion Strong Regionally restricted euxassay_009463_05
TS23: telencephalon mantle layer Strong Regionally restricted euxassay_009463_05
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_05
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_05
Specimen euxassay_009463_06: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_06
TS23: vestibulo-cochlear ganglion Strong Regionally restricted euxassay_009463_06
TS23: glossopharyngeal ganglion Strong Regionally restricted euxassay_009463_06
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_06
TS23: telencephalon mantle layer Strong Regionally restricted euxassay_009463_06
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_06
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_06
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_06
TS23: lower lip Strong Regionally restricted euxassay_009463_06
Specimen euxassay_009463_07: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: glossopharyngeal ganglion Strong Regionally restricted euxassay_009463_07
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_07
TS23: vestibulo-cochlear ganglion Strong Regionally restricted euxassay_009463_07
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_07
TS23: vagus ganglion Strong Regionally restricted euxassay_009463_07
TS23: midgut Strong Regionally restricted euxassay_009463_07
TS23: telencephalon mantle layer Strong Regionally restricted euxassay_009463_07
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_07
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_07
TS23: pons Strong Regionally restricted euxassay_009463_07
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_07
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_07
TS23: lower lip Strong Regionally restricted euxassay_009463_07
Specimen euxassay_009463_08: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_08
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_08
TS23: cervico-thoracic ganglion Strong Regionally restricted euxassay_009463_08
TS23: midgut Strong Regionally restricted euxassay_009463_08
TS23: diencephalon lateral wall mantle layer Moderate Regionally restricted euxassay_009463_08
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_08
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_08
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_08
TS23: pons Strong Regionally restricted euxassay_009463_08
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_08
TS23: spinal cord mantle layer Strong Regionally restricted euxassay_009463_08
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_08
Specimen euxassay_009463_09: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_09
TS23: thymus primordium Weak Regionally restricted euxassay_009463_09
TS23: midgut Weak Regionally restricted euxassay_009463_09
TS23: diencephalon lateral wall mantle layer Moderate Regionally restricted euxassay_009463_09
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_09
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_09
TS23: pons Strong Regionally restricted euxassay_009463_09
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_09
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_09
TS23: spinal cord mantle layer Strong Regionally restricted euxassay_009463_09
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_09
Specimen euxassay_009463_10: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_10
TS23: thoracic ganglion Strong Regionally restricted euxassay_009463_10
TS23: thymus primordium Weak Regionally restricted euxassay_009463_10
TS23: midgut Weak Regionally restricted euxassay_009463_10
TS23: diencephalon lateral wall mantle layer Strong Regionally restricted euxassay_009463_10
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_10
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_10
TS23: pons Strong Regionally restricted euxassay_009463_10
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_10
TS23: spinal cord mantle layer Strong Regionally restricted euxassay_009463_10
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_10
Specimen euxassay_009463_11: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: thoracic ganglion Strong Regionally restricted euxassay_009463_11
TS23: midgut Moderate Regionally restricted euxassay_009463_11
TS23: diencephalon lateral wall mantle layer Strong Regionally restricted euxassay_009463_11
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_11
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_11
TS23: pons Strong Regionally restricted euxassay_009463_11
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_11
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_11
TS23: spinal cord mantle layer Strong Regionally restricted euxassay_009463_11
Specimen euxassay_009463_12: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: cervico-thoracic ganglion Strong Regionally restricted euxassay_009463_12
TS23: thoracic ganglion Strong Regionally restricted euxassay_009463_12
TS23: thymus primordium Weak Regionally restricted euxassay_009463_12
TS23: midgut Moderate Regionally restricted euxassay_009463_12
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_12
TS23: diencephalon lateral wall mantle layer Moderate Regionally restricted euxassay_009463_12
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_12
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_12
TS23: spinal cord mantle layer Strong Regionally restricted euxassay_009463_12
Specimen euxassay_009463_13: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_13
TS23: cervico-thoracic ganglion Strong Regionally restricted euxassay_009463_13
TS23: thymus primordium Weak Regionally restricted euxassay_009463_13
TS23: midgut Moderate Regionally restricted euxassay_009463_13
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_13
TS23: diencephalon lateral wall mantle layer Moderate Regionally restricted euxassay_009463_13
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_13
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_13
TS23: pons Strong Regionally restricted euxassay_009463_13
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_13
TS23: spinal cord mantle layer Strong Regionally restricted euxassay_009463_13
Specimen euxassay_009463_14: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_14
TS23: midgut Moderate Regionally restricted euxassay_009463_14
TS23: diencephalon lateral wall mantle layer Moderate Regionally restricted euxassay_009463_14
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_14
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_14
TS23: pons Strong Regionally restricted euxassay_009463_14
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_14
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_14
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_14
Specimen euxassay_009463_15: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: dorsal root ganglion Strong Regionally restricted euxassay_009463_15
TS23: vagus ganglion Strong Regionally restricted euxassay_009463_15
TS23: hindgut Moderate Regionally restricted euxassay_009463_15
TS23: diencephalon lateral wall mantle layer Strong Regionally restricted euxassay_009463_15
TS23: telencephalon mantle layer Strong Regionally restricted euxassay_009463_15
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_15
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_15
TS23: pons Strong Regionally restricted euxassay_009463_15
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_15
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_15
Specimen euxassay_009463_16: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: glossopharyngeal ganglion Strong Regionally restricted euxassay_009463_16
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_16
TS23: vestibulo-cochlear ganglion Strong Regionally restricted euxassay_009463_16
TS23: diencephalon lateral wall mantle layer Moderate Regionally restricted euxassay_009463_16
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_16
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_16
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_16
TS23: pons Strong Regionally restricted euxassay_009463_16
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_16
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_16
TS23: lower lip Moderate Regionally restricted euxassay_009463_16
Specimen euxassay_009463_17: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_17
TS23: vestibulo-cochlear ganglion Strong Regionally restricted euxassay_009463_17
TS23: glossopharyngeal ganglion Strong Regionally restricted euxassay_009463_17
TS23: telencephalon mantle layer Moderate Regionally restricted euxassay_009463_17
TS23: diencephalon lateral wall mantle layer Moderate Regionally restricted euxassay_009463_17
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_17
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_17
TS23: pons Strong Regionally restricted euxassay_009463_17
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_17
TS23: medulla oblongata basal plate Strong Regionally restricted euxassay_009463_17
TS23: lower lip Strong Regionally restricted euxassay_009463_17
Specimen euxassay_009463_18: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_18
TS23: facial ganglion Strong Regionally restricted euxassay_009463_18
TS23: telencephalon mantle layer Strong Regionally restricted euxassay_009463_18
TS23: midbrain mantle layer Strong Regionally restricted euxassay_009463_18
TS23: pons Strong Regionally restricted euxassay_009463_18
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_18
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_18
TS23: lower lip Weak Regionally restricted euxassay_009463_18
Specimen euxassay_009463_19: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_19
TS23: facial ganglion Strong Regionally restricted euxassay_009463_19
TS23: telencephalon mantle layer Strong Regionally restricted euxassay_009463_19
TS23: rest of cerebellum marginal layer Strong Regionally restricted euxassay_009463_19
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_19
Specimen euxassay_009463_20: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS23: trigeminal ganglion Strong Regionally restricted euxassay_009463_20
TS23: facial ganglion Strong Regionally restricted euxassay_009463_20
TS23: telencephalon mantle layer Strong Regionally restricted euxassay_009463_20
TS23: rest of cerebellum mantle layer Strong Regionally restricted euxassay_009463_20
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
12/30/2025
MGI 6.24 		The Jackson Laboratory