Summary | Transgene origin | Transgene description | Recombinase activity | Phenotypes | Tumor data | Find Mice (IMSR) | References

Symbol:	Tg(Cspg4-cre/Esr1*)BAkik
Name:	transgene insertion B, Akiko Nishiyama
MGI ID:	MGI:4819178
Synonyms:	NG2-CreER, NG2-CreER, NG2creERB, NG2creERTM, NG2creERTM
Transgene:	Tg(Cspg4-cre/Esr1*)BAkik  Location: Chr14:106892213-106892841 bp  Genetic Position: Chr14, Syntenic
Alliance:	Tg(Cspg4-cre/Esr1*)BAkik page

Strain of Origin:

C57BL/6 or (C57BL/6 x SJL)F1

Transgene Type:    Transgenic (Inducible, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intergenic deletion   Tg(Cspg4-cre/Esr1*)BAkik expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Cspg4 (MGI:2153093)   Mutation details: The 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), was modified by inserting a CreERTM fusion gene (cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand-binding domain) followed by a rabbit beta-globin polyA signal, into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent the translation from the BAC NG2 gene and to allow translation from the first ATG in the CreERTM sequence. The ~219 kb BAC sequence was linearized and microinjected into the pronucleus of fertilized oocytes from C57BL/6 or (C57BL/6 x SJL)F1 mice. Mice derived from line B (NG2creERB) had no background cre activity in the absence of tamoxifen, with cre activity specifically in NG2-expressing cells after tamoxifen injection. Transgene insertion occurred on Chr 14, causing a 628 bp deletion. (J:162529, J:167902)

Activity:	Tissue activity of this recombinase allele
Driver:	Cspg4 (mouse) Summary of all recombinase alleles driven by Cspg4. Comparative matrix view of recombinase activities Alliance mouse Cspg4 gene page

View phenotypes and curated references for all genotypes (concatenated display).

List all tumor models in MMHCdb carrying Tg(Cspg4-cre/Esr1*)BAkik

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:167902 Zhu X, et al., Age-dependent fate and lineage restriction of single NG2 cells. Development. 2011 Feb;138(4):745-53
All:	115 reference(s)