Probe:	Sulf1_#14
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		1O
TS20: trunk mesenchyme	Present	Regionally restricted	1O	Expression was detected in the mesenchyme surrounding the cartilage primordia of the vertebrae.

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		1P
TS22: brainstem	Absent		1P

Structure	Level	Pattern	Image	Note
TS20: spinal cord floor plate	Present		1U
TS20: trunk mesenchyme	Present	Regionally restricted	1U	Expression was detected in the mesenchyme surrounding the cartilage primordia of the vertebrae.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		1V

Structure	Level	Pattern	Image	Note
TS20: visceral pleura	Present	Regionally restricted	2A	Expression was detected in the pleural lining of the lung.
TS20: gut mesentery	Present		2A
TS20: stomach mesentery	Present		2A

Structure	Level	Pattern	Image	Note
TS22: visceral pleura	Present	Regionally restricted	2B	Expression was detected in the pleural lining of the lung.
TS22: esophagus	Present	Regionally restricted	2B	Expression was detected in the muscle progenitors.
TS22: dorsal aorta	Present	Regionally restricted	2B	Expression was detected in the aorta wall.
TS22: pericardium	Present		2B
TS22: visceral pericardium	Present		2B

Structure	Level	Pattern	Image	Note
TS22: esophagus	Present		2F
TS22: stomach	Present		2F
TS22: ureter	Present		2F

Structure	Level	Pattern	Image	Note
TS22: gonad	Present		2G

Structure	Level	Pattern	Image	Note
TS22: stomach	Present		2H
TS22: spleen primordium	Present	Regionally restricted	2H	Expression was detected in the splenic mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present		3E
TS22: telencephalon	Present	Regionally restricted	3E	Expression was detected in the cortical hem.
TS22: head mesenchyme	Present	Regionally restricted	3E	Expression was detected surrounding the parachordal plate (future basioccipital and basisphenoid bones).
TS22: Meckel's cartilage	Present		3E
TS22: cochlear duct epithelium	Present		3E

Structure	Level	Pattern	Image	Note
TS22: spinal cord floor plate	Present		3I

Structure	Level	Pattern	Image	Note
TS22: lens epithelium	Present		4E
TS22: cornea	Absent		4E
TS22: lens fiber	Absent		4E
TS22: perioptic mesenchyme	Present		4E

Structure	Level	Pattern	Image	Note
TS24: lens epithelium	Present		4K
TS24: cornea	Absent		4K
TS24: lens fiber	Absent		4K
TS24: perioptic mesenchyme	Present		4K

Structure	Level	Pattern	Image	Note
TS22: elbow joint primordium	Present	Regionally restricted	5E	Expression was detected in articular chondrocytes of the elbow joint.

Structure	Level	Pattern	Image	Note
TS24: ulna	Present	Regionally restricted	5K	Expression was detected in hypertrophic chondrocytes and in the developing bone.
TS24: elbow joint	Absent		5K

Structure	Level	Pattern	Image	Note
TS20: limb digit	Present		6A

Structure	Level	Pattern	Image	Note
TS21: limb digit	Present		6B

Structure	Level	Pattern	Image	Note
TS22: forelimb digit	Present	Regionally restricted	6G	Expression was detected in the tip of the digits.

Structure	Level	Pattern	Image	Note
TS22: radius cartilage condensation	Present		6H