Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo
Transgene Detail
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| Symbol: |
Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo |
| Name: |
transgene insertion 1.1, Liqun Luo |
| MGI ID: |
MGI:4461409 |
| Synonyms: |
TRE-Bi-SG-T line 1.1 |
| Transgene: |
Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo Location: unknown
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| Alliance: |
Tg(tetO-tdTomato,-Syp/EGFP*)1.1Luo page
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| Transgene Type: |
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Transgenic (Inducible, Inserted expressed sequence, Reporter) |
| Inducer: |
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doxycycline/tetracycline |
| Mutation: |
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Insertion
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Mutation details: The TRE-Bi-SG-T transgene was designed with a tdTomato protein sequence on one side, and a Syp-mut4EGFP fusion protein sequence on the other side of a bi-directional tetracycline-responsive promoter (tetO; also called tetracycline operator, tet-operator, or tetracycline-responsive element [TRE]) with CMV minimal enhancer-less promoter. The tdTomato is tagged with three Myc epitopes at its C-terminus and followed by a polyadenylation signal (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)). The Syp-mut4EGFP fusion protein is a full-length mouse synaptophysin (Syp) fused to a mutant enhanced green fluorescent protein (mut4EGFP; harboring the V163A/S175G codon changes for increased ability to fold properly at 37 degrees C) and followed by a polyadenylation signal.
(J:161840)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:161840 Li L, et al., Visualizing the distribution of synapses from individual neurons in the mouse brain. PLoS One. 2010;5(7):e11503 |
| All: |
14 reference(s) |
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Analysis Tools
