Antibody:	PAK1 (C19) antibody
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	This antibody recognizes Pak1-3.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Strong		1A
TS22: cortical plate	Strong		1A
TS22: cerebral cortex subventricular zone	Present		1A
TS22: cerebral cortex ventricular layer	Present		1A

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Widespread	1B
TS23: cerebral cortex intermediate zone	Strong	Regionally restricted	1B	Expression was high in axonal fibers.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Widespread	1C	Expression in this mutant is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Strong		1D
TS25: cerebral cortex ventricular zone	Present		1D
TS25: cortical plate	Present		1D

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		1E
TS28: cortical layer IV	Present		1E
TS28: cortical layer III	Present		1E	The highest level of expression is in pyramidal cell soma and processes in layers III and V.
TS28: cortical layer V	Present		1E	The highest level of expression is in pyramidal cell soma and processes in layers III and V.
TS28: cortical layer VI	Present		1E	A somewhat lower level in layer VI.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex intermediate zone	Strong		1J merge , 1J PAK1-3
TS23: cerebral cortex subventricular zone	Present		1J PAK1-3 , 1J merge

Structure	Level	Pattern	Image	Note
TS28: neocortex layer	Present			Expression in this mutant is the same as in wild-type in layers II-VI, with highest level of expression in pyramidal cell soma and processes in layers III and V, and somewhat lower levels in layer VI.