Probe:	pSP65
Probe preparation:	Antisense labelled with tritium (H3) RNA

Structure	Level	Pattern	Image	Note
TS16: telencephalon	Present	Regionally restricted	2a	Expression in the tela choroidea.

Structure	Level	Pattern	Image	Note
TS16: yolk sac endoderm	Present		2b	Expression in endodermal cells of the visceral yolk sac.

Structure	Level	Pattern	Image	Note
TS16: hepatic primordium	Present		2c	Authors report small but significant expression in hepatocytes.

Structure	Level	Pattern	Image	Note
TS20: choroid plexus	Strong	Regionally restricted	2d	High levels of expression in choroid plexus epithelial cells.

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Regionally restricted	2e	Expression in almost all hepatocytes. No expression in hematopoietic cells of the fetal liver.

Structure	Level	Pattern	Image	Note
TS20: labyrinthine zone	Absent			No expression in the labyrinth region of the placenta, splanchnic mesodermal cells or blood islands of the yolk sac, or the amnion.

Structure	Level	Pattern	Image	Note
TS20: yolk sac endoderm	Present	Regionally restricted	3a	Expression in endodermal cells of the visceral yolk sac. Greater expression in the region proximal to the placenta than distally.

Structure	Level	Pattern	Image	Note
TS20: yolk sac endoderm	Present	Regionally restricted	3b	Expression in endodermal cells of the visceral yolk sac. Greater expression in the region proximal to the placenta than distally.

Structure	Level	Pattern	Image	Note
TS23: choroid plexus	Present	Regionally restricted	4a	Uniform expression in the cytoplasm of choroid plexus epithelial cells.

Structure	Level	Pattern	Image	Note
TS23: liver	Present		4b	Uniform expression in the cytoplasm of almost all hepatocytes.

Structure	Level	Pattern	Image	Note
TS23: brain ventricular layer	Absent			Authors report only background levels of expression were detected in other regions of the brain, such as cerebral cortex, medulla, and ependymal lining of the ventricles.
TS23: cerebral cortex	Absent			Authors report only background levels of expression were detected in other regions of the brain, such as cerebral cortex, medulla, and ependymal lining of the ventricles.
TS23: medulla oblongata	Absent			Authors report only background levels of expression were detected in other regions of the brain, such as cerebral cortex, medulla, and ependymal lining of the ventricles.

Structure	Level	Pattern	Image	Note
TS25: choroid plexus	Present	Regionally restricted	4c	Uniform expression in the cytoplasm of choroid plexus epithelial cells.

Structure	Level	Pattern	Image	Note
TS25: liver	Present		4d	Uniform expression in the cytoplasm of almost all hepatocytes.

Structure	Level	Pattern	Image	Note
TS25: brain ventricular layer	Absent			Authors report only background levels of expression were detected in other regions of the brain, such as cerebral cortex, medulla, and ependymal lining of the ventricles.
TS25: cerebral cortex	Absent			Authors report only background levels of expression were detected in other regions of the brain, such as cerebral cortex, medulla, and ependymal lining of the ventricles.
TS25: medulla oblongata	Absent			Authors report only background levels of expression were detected in other regions of the brain, such as cerebral cortex, medulla, and ependymal lining of the ventricles.

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Strong	Regionally restricted	5b	Strong expression specifically in choroid epithelial cells.

Structure	Level	Pattern	Image	Note
TS28: liver	Present		5c	Unifrom expression throughout the cytoplasm of almost all hepatocytes.

Structure	Level	Pattern	Image	Note
TS28: metanephros	Absent