Probe:	UI-M-BH3-atm-f-07-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Strong	Non-Uniform	Zswim6-Shh E11.5_coronal	Staining is strong in the prethalamus, moderate in anterior & posterior ID, moderate & scattered in dorsal POA, weak in PVH nucleus & either absent or ubiquitous in dorsal thalamus, ventral POA, AVH, VMH & ARH nuclei, premam, mam & supramam zones & TT.
TS19: diencephalon lateral wall ventricular layer	Ambiguous		Zswim6-Shh E11.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer	Strong	Non-Uniform	Zswim6-Shh E11.5_coronal	There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer	Ambiguous		Zswim6-Shh E11.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Zswim6-Shh E12.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Zswim6-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Zswim6-Shh E12.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Strong	Non-Uniform	Zswim6-Shh E12.5_coronal	There is strong staining in the mantle zone of the TT. Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS20: diencephalon ventricular layer	Ambiguous		Zswim6-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Very strong	Non-Uniform	Zswim6-Shh E12.5_coronal	There is very strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	Zswim6-Shh E12.5_coronal	There is strong staining in the MZ of the anterior and posterior IDs, weak staining in the PVH nucleus and weak, scattered staining in the dorsal and ventral POAs. Staining is either absent or ubiquitous in the MZ of the AVH and the VMH and ARH nuclei.
TS20: hypothalamus ventricular layer	Ambiguous		Zswim6-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Strong	Non-Uniform	Zswim6-Shh E12.5_coronal	There is strong staining in the mantle zone of the prethalamus and weak staining in the dorsal thalamus.
TS20: thalamus ventricular layer	Ambiguous		Zswim6-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Zswim6-Shh E14.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Zswim6-Shh E14.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Zswim6-Shh E14.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Very strong	Non-Uniform	Zswim6-Shh E14.5_coronal	There is very strong staining in the mantle zone of the TT. Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS22: diencephalon ventricular layer	Ambiguous		Zswim6-Shh E14.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Weak	Non-Uniform	Zswim6-Shh E14.5_coronal	There is weak staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Weak	Non-Uniform	Zswim6-Shh E14.5_coronal	There is weak staining in the mantle zone of the PVH nucleus and the AVH. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the VMH and ARH nuclei and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Ambiguous		Zswim6-Shh E14.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Strong	Non-Uniform	Zswim6-Shh E14.5_coronal	There is strong staining in the mantle zone of the prethalamus and weak staining in the dorsal thalamus.
TS22: thalamus ventricular layer	Ambiguous		Zswim6-Shh E14.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Strong		Zswim6-Shh E16.5_coronal	There is strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Zswim6-Shh E16.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Strong		Zswim6-Shh E16.5_coronal	There is strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: ganglionic eminence	Moderate	Non-Uniform	Zswim6-Shh E16.5_coronal	There is moderate staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Weak	Non-Uniform	Zswim6-Shh E16.5_coronal	There is weak staining in the mantle zone of the DAH. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH and DMH nuclei, the AVH and the PH.
TS24: hypothalamus ventricular layer	Ambiguous		Zswim6-Shh E16.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH and DMH nuclei, the AVH and the PH.
TS24: thalamus mantle layer	Strong	Non-Uniform	Zswim6-Shh E16.5_coronal	There is strong staining in the mantle zone of the prethalamus and weak staining in the dorsal thalamus.
TS24: thalamus ventricular layer	Weak	Non-Uniform	Zswim6-Shh E16.5_coronal	There is weak staining in the ventricular zone of the dorsal thalamus. Staining is either absent or ubiquitous in the ventricular zone of the prethalamus.