Probe:	UI-M-AK0-add-c-01-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Moderate	Non-Uniform	Odz2-Shh E11.5 sagittal , Odz2-Shh E11.5 coronal	Staining is moderate in the MZ of the prethalamus, dorsal & ventral POAs, PVH & VMH nuclei & mam & supramam zones, weak in the dorsal thalamus, anterior & posterior IDs & TT & either absent or ubiquitous in the AVH, ARH nucleus & premam zone.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Odz2-Shh E11.5 sagittal , Odz2-Shh E11.5 coronal	There is strong staining in the VZ of the dorsal thalamus. Staining is either absent or ubiquitous in the VZ of the prethalamus, dorsal & ventral POAs, the PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam, mam & supramam zones & the TT.
TS19: telencephalon mantle layer	Weak	Non-Uniform	Odz2-Shh E11.5 sagittal , Odz2-Shh E11.5 coronal	There is weak staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex.
TS19: telencephalon ventricular layer	Weak	Non-Uniform	Odz2-Shh E11.5 coronal , Odz2-Shh E11.5 sagittal	There is weak staining in the ventricular zone of the cerebral cortex. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Moderate		Odz2-Shh E12.5 sagittal , Odz2-Shh E12.5 coronal	There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Odz2-Shh E12.5 sagittal , Odz2-Shh E12.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Moderate		Odz2-Shh E12.5 coronal , Odz2-Shh E12.5 sagittal	There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Strong	Non-Uniform	Odz2-Shh E12.5 coronal , Odz2-Shh E12.5 sagittal	There is strong staining in the mantle zone of the premam and mam zones, moderate staining in the TT and weak staining in the supramam zone.
TS20: diencephalon ventricular layer	Ambiguous		Odz2-Shh E12.5 coronal , Odz2-Shh E12.5 sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence	Weak	Non-Uniform	Odz2-Shh E12.5 coronal , Odz2-Shh E12.5 sagittal	There is weak staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	Odz2-Shh E12.5 sagittal , Odz2-Shh E12.5 coronal	There is strong staining in the mantle zone of the PVH nucleus, anterior and posterior IDs and VMH nucleus and moderate staining in the ventral POA. Staining is either absent or ubiquitous in the mantle zone of the dorsal POA, the AVH and ARH nucleus.
TS20: hypothalamus ventricular layer	Ambiguous		Odz2-Shh E12.5 sagittal , Odz2-Shh E12.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer	Strong		Odz2-Shh E12.5 sagittal , Odz2-Shh E12.5 coronal	There is strong staining in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Odz2-Shh E12.5 coronal , Odz2-Shh E12.5 sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Moderate		Odz2-Shh E14.5 coronal , Odz2-Shh E14.5 sagittal	There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Odz2-Shh E14.5 sagittal , Odz2-Shh E14.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Moderate		Odz2-Shh E14.5 sagittal , Odz2-Shh E14.5 coronal	There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Very strong	Non-Uniform	Odz2-Shh E14.5 sagittal , Odz2-Shh E14.5 coronal	There is very strong staining in the mantle zone of the mam zone, moderate staining in the TT and weak staining in the premam and supramam zones.
TS22: diencephalon ventricular layer	Ambiguous		Odz2-Shh E14.5 sagittal , Odz2-Shh E14.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence	Moderate	Non-Uniform	Odz2-Shh E14.5 sagittal , Odz2-Shh E14.5 coronal	There is moderate staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS22: hypothalamus mantle layer	Strong	Non-Uniform	Odz2-Shh E14.5 coronal , Odz2-Shh E14.5 sagittal	There is strong staining in the MZ of the VMH nucleus, moderate staining in the PVH nucleus, the AVH and anterior and posterior IDs and weak staining in the dorsal POA. Staining is either absent or ubiquitous in the MZ of the ventral POA and ARH nucleus.
TS22: hypothalamus ventricular layer	Ambiguous		Odz2-Shh E14.5 coronal , Odz2-Shh E14.5 sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer	Very strong	Non-Uniform	Odz2-Shh E14.5 sagittal , Odz2-Shh E14.5 coronal	There is very strong staining in the mantle zone of the dorsal thalamus and strong staining in the prethalamus.
TS22: thalamus ventricular layer	Strong	Non-Uniform	Odz2-Shh E14.5 sagittal , Odz2-Shh E14.5 coronal	There is strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Strong		Odz2-Shh E16.5 sagittal , Odz2-Shh E16.5 coronal	There is strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Strong		Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	There is strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Very strong	Non-Uniform	Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	There is very strong staining in the mantle zone of the mam zone and weak staining in the supramam zone. Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS24: diencephalon ventricular layer	Ambiguous		Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence	Strong	Non-Uniform	Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	There is strong staining in the mantle zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.
TS24: hypothalamus mantle layer	Very strong	Non-Uniform	Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	Staining is very strong in the MZ of the VMH nucleus, strong in the AVH, moderate in the PVH nucleus & weak in the DMH nucleus & PH. Staining is either absent or ubiquitous in the MZ of the dorsal & ventral POAs, DAH & SCH & ARH nuclei.
TS24: hypothalamus ventricular layer	Ambiguous		Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer	Very strong	Non-Uniform	Odz2-Shh E16.5 coronal , Odz2-Shh E16.5 sagittal	There is very strong staining in the mantle zone of the dorsal thalamus and strong staining in the prethalamus.
TS24: thalamus ventricular layer	Strong	Non-Uniform	Odz2-Shh E16.5 sagittal , Odz2-Shh E16.5 coronal	There is strong staining in the ventricular zone of the prethalamus and weak staining in the dorsal thalamus.