Probe:	UI-R-DK0-cfh-a-03-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_f	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_g	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_h	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_i	There is broad, selective expression in hypothalamic progenitors, as well as in a subset of postmitotic cells in the intrahypothalamic diagonal.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_j	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS28: dorsomedial hypothalamic nucleus	Present			Expression is detected in neurons.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Gpr50-Shh E11.5 coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Very strong	Non-Uniform	Gpr50-Shh E11.5 coronal	Staining is very strong in the VZ of the AVH, anterior & posterior IDs, VMH & ARH nuclei, premam zone & TT, moderate in dorsal & ventral POAs & PVH nucleus & either absent or ubiquitous in the dorsal thalamus, prethalamus, mam & supramam zones.
TS19: telencephalon mantle layer	Ambiguous		Gpr50-Shh E11.5 coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Moderate	Non-Uniform	Gpr50-Shh E11.5 coronal	There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Gpr50-Shh E12.5 coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Gpr50-Shh E12.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Gpr50-Shh E12.5 coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Gpr50-Shh E12.5 coronal	Staining is either absent or ubiquitous in the mantle zone of the premam zone and the TT.
TS20: diencephalon ventricular layer	Very strong		Gpr50-Shh E12.5 coronal	There is very strong staining in the ventricular zone of the premam zone and the TT.
TS20: ganglionic eminence	Ambiguous		Gpr50-Shh E12.5 coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Moderate	Non-Uniform	Gpr50-Shh E12.5 coronal	There is moderate staining in the mantle zone of the ventral POA. Staining is either absent or ubiquitous in the mantle zone of the dorsal POA, the PVH and VMH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Very strong	Non-Uniform	Gpr50-Shh E12.5 coronal	There is very strong staining in the ventricular zone of the anterior and posterior IDs and VMH nucleus and moderate staining in the PVH nucleus and AVH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs.
TS20: thalamus mantle layer	Ambiguous		Gpr50-Shh E12.5 coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Gpr50-Shh E12.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Gpr50-Shh E14.5 coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Gpr50-Shh E14.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Gpr50-Shh E14.5 coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Gpr50-Shh E14.5 coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Strong	Non-Uniform	Gpr50-Shh E14.5 coronal	There is strong staining in the ventricular zone of the premam and mam zones and the TT. Staining is either absent or ubiquitous in the ventricular zone of supramam zone.
TS22: ganglionic eminence	Ambiguous		Gpr50-Shh E14.5 coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Strong	Non-Uniform	Gpr50-Shh E14.5 coronal	There is strong staining in the mantle zone of the anterior ID. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and posterior ID.
TS22: hypothalamus ventricular layer	Strong	Non-Uniform	Gpr50-Shh E14.5 coronal	There is strong staining in the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs.
TS22: thalamus mantle layer	Ambiguous		Gpr50-Shh E14.5 coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Ambiguous		Gpr50-Shh E14.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Gpr50-Shh E16.5 coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Gpr50-Shh E16.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Gpr50-Shh E16.5 coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Gpr50-Shh E16.5 coronal	Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS24: diencephalon ventricular layer	Very strong		Gpr50-Shh E16.5 coronal	There is very strong staining in the ventricular zone of the premam zone.
TS24: ganglionic eminence	Ambiguous		Gpr50-Shh E16.5 coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Very strong	Non-Uniform	Gpr50-Shh E16.5 coronal	Staining is very strong in the MZ of the DMH nucleus, strong in the PH, moderate in the PVH nucleus & weak in the ventral POA. Staining is either absent or ubiquitous in the MZ of the dorsal POA, AVH, DAH & SCH, VMH & ARH nuclei.
TS24: hypothalamus ventricular layer	Very strong	Non-Uniform	Gpr50-Shh E16.5 coronal	There is very strong staining in the VZ of the AVH and the SCH, DMH and VMH nuclei and moderate staining in the ARH nucleus. Staining is either absent or ubiquitous in the VZ of the dorsal and ventral POAs, the PVH nucleus, the DAH and the PH.
TS24: thalamus mantle layer	Ambiguous		Gpr50-Shh E16.5 coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Ambiguous		Gpr50-Shh E16.5 coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.