Probe:	Rmst cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Strong		Dmt2-Shh E11.5-coronal	There is strong staining in the mantle zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH nucleus and the AVH.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Dmt2-Shh E11.5-coronal	There is strong staining in the ventricular zone of the prethalamus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, dorsal and ventral POAs, the PVH nucleus and the AVH.
TS19: telencephalon mantle layer	Strong	Non-Uniform	Dmt2-Shh E11.5-coronal	There is strong staining in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and weak staining in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Strong	Non-Uniform	Dmt2-Shh E11.5-coronal	There is strong staining in the ventricular zone of the cerebral cortex. Staining is either absent or ubiquitous in the ventricular zone of the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Very strong		Dmt2-Shh E12.5-coronal	There is very strong staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Very strong		Dmt2-Shh E12.5-coronal	There is very strong staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Very strong		Dmt2-Shh E12.5-coronal	There is very strong staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Very strong	Non-Uniform	Dmt2-Shh E12.5-coronal	There is very strong staining in the mantle zone of the mam zone and weak staining in the TT. Staining is either absent or ubiquitous in the mantle zone of the premam and supramam zones.
TS20: diencephalon ventricular layer	Very strong	Non-Uniform	Dmt2-Shh E12.5-coronal	There is very strong staining in the ventricular zone of the mam zone and moderate staining in the TT. Staining is either absent or ubiquitous in the ventricular zone of the premam and supramam zones.
TS20: ganglionic eminence	Ambiguous		Dmt2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Strong	Non-Uniform	Dmt2-Shh E12.5-coronal	There is strong staining in the mantle zone of the PVH nucleus and the AVH and weak staining in the anterior and posterior IDs. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs and the VMH and ARH nuclei.
TS20: hypothalamus ventricular layer	Strong	Non-Uniform	Dmt2-Shh E12.5-coronal	There is strong staining in the ventricular zone of the dorsal and ventral POAs and moderate staining in the anterior and posterior IDs. Staining is either absent or ubiquitous in the ventricular zone of the PVH, VMH and ARH nuclei and the AVH.
TS20: thalamus mantle layer	Strong		Dmt2-Shh E12.5-coronal	There is strong staining in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Dmt2-Shh E12.5-coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Dmt2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Very strong		Dmt2-Shh E14.5-coronal	There is very strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Dmt2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Dmt2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Very strong	Non-Uniform	Dmt2-Shh E14.5-coronal	There is very strong staining in the ventricular zone of the TT and mam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam and supramam zones.
TS22: ganglionic eminence	Ambiguous		Dmt2-Shh E14.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Weak	Non-Uniform	Dmt2-Shh E14.5-coronal	There is weak staining in the mantle zone of the ventral POA, the PVH nucleus and the AVH. Staining is either absent or ubiquitous in the mantle zone of the dorsal POA, anterior and posterior IDs and VMH and ARH nuclei.
TS22: hypothalamus ventricular layer	Strong	Non-Uniform	Dmt2-Shh E14.5-coronal	There is strong staining in the ventricular zone of the ventral POA and the AVH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal POA, PVH, VMH and ARH nuclei and anterior and posterior IDs.
TS22: thalamus mantle layer	Moderate	Non-Uniform	Dmt2-Shh E14.5-coronal	There is moderate staining in the mantle zone of the dorsal thalamus and weak staining in the prethalamus.
TS22: thalamus ventricular layer	Moderate		Dmt2-Shh E14.5-coronal	There is moderate staining in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Dmt2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Moderate		Dmt2-Shh E16.5-coronal	There is moderate staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Dmt2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Weak	Non-Uniform	Dmt2-Shh E16.5-coronal	There is weak staining in the mantle zone of the premam zone. Staining is either absent or ubiquitous in the mantle zone of the mam and supramam zones.
TS24: diencephalon ventricular layer	Very strong	Non-Uniform	Dmt2-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the premam and mam zones. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS24: ganglionic eminence	Ambiguous		Dmt2-Shh E16.5-coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Strong	Non-Uniform	Dmt2-Shh E16.5-coronal	There is strong staining in the MZ of the PVH nucleus, moderate staining in the DAH and PH and weak staining in the dorsal and ventral POAs and the DMH, VMH and ARH nuclei. Staining is either absent or ubiquitous in the MZ of the AVH and SCH nucleus.
TS24: hypothalamus ventricular layer	Very strong	Non-Uniform	Dmt2-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the dorsal and ventral POAs, the SCH, DMH, VMH and ARH nuclei and the PH and moderate staining in the PVH nucleus, the AVH and the DAH.
TS24: thalamus mantle layer	Moderate	Non-Uniform	Dmt2-Shh E16.5-coronal	There is moderate staining in the mantle zone of the dorsal thalamus and weak staining in the prethalamus.
TS24: thalamus ventricular layer	Very strong		Dmt2-Shh E16.5-coronal	There is very strong staining in the ventricular zone of the dorsal thalamus and prethalamus.