Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Muc1tm1(cre/ERT2)Lcm
Name:	mucin 1, transmembrane; targeted mutation 1, Lewis Murtaugh
MGI ID:	MGI:4443061
Synonyms:	Muc1IC2neo, Muc1IRES-CreERT2-neo
Gene:	Muc1  Location: Chr3:89136364-89140688 bp, + strand  Genetic Position: Chr3, 39.02 cM
Alliance:	Muc1tm1(cre/ERT2)Lcm page

Germline Transmission:	Earliest citation of germline transmission: J:159445
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Muc1tm1(cre/ERT2)Lcm expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Muc1 (MGI:97231)   Mutation details: Downstream of the translation termination codon of the endogenous gene was inserted an internal ribosomal entry site (IRES) followed by DNA encoding Cre recombinase joined to ERT2 and a frt site-flanked neomycin resistance cassette (IRES-CreERT2-FRT-neoR-FRT). ERT2 is a triply mutated (G400V, M543A, L544A) human estrogen receptor ligand-binding domain (LBD) that renders the fusion protein highly sensitive to activation by 4-hydroxytamoxifen, but unresponsive to the receptor's native ligands. (J:159445)

Generation of the Muc1tm1(cre/ERT2)Lcm allele

Activity:	Tissue activity of this recombinase allele
Driver:	Muc1 (mouse) Summary of all recombinase alleles driven by Muc1. Alliance mouse Muc1 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Muc1 Mutation:	37 strains or lines available

Original:	J:159445 Kopinke D, et al., Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas. BMC Dev Biol. 2010;10:38
All:	1 reference(s)