Gene Expression Data
Assay Details
Assay
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Reference:
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J:21265
Dolle P, et al., Coordinate expression of the murine Hox-5 complex homoeobox-containing genes during limb pattern formation. Nature. 1989 Dec 14;342(6251):767-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4442509
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Gene symbol:
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Hoxd9
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Gene name:
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homeobox D9
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Probe:
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Hox-5.2 probe1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
not shown:
embryonic day 8.75
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS13: trunk mesenchyme |
Present |
Regionally restricted |
|
Expression in the forelimb field before limb bud outgrowth.
|
Specimen
1a left:
embryonic day 9.25
(more )
Specimen
1a left:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: forelimb bud mesenchyme |
Strong |
Regionally restricted |
1a left Hox-5.2
|
Strong expression in the limb bud mesoderm, with a sharp expression boundary.
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Specimen
1a right:
embryonic day 9.25
(more )
Specimen
1a right:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.25
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: forelimb bud mesenchyme |
Strong |
Regionally restricted |
1a right Hox-5.2
|
Strong expression in the limb bud mesoderm, with a sharp expression boundary.
|
Specimen
1b:
embryonic day 9.75
(more )
Specimen
1b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: forelimb bud mesenchyme |
Present |
Regionally restricted |
1b Hox-5.2
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Expression in proximal limb mesoderm, with a distinct proximal expression boundary.
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Specimen
1c:
embryonic day 10.5
(more )
Specimen
1c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
1c Hox-5.2
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Expression in proximal limb mesoderm, with a distinct proximal expression boundary.
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Specimen
1d:
embryonic day 12.5
(more )
Specimen
1d:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb pre-cartilage condensation |
Strong |
Regionally restricted |
1d Hox-5.2
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Expression restricted to central limb precartilage condensations. Strong expression in proximal humeral head blastema. Weaker expression in the posterior half of the forelimb tip.
|
Specimen
1e:
embryonic day 12.5
(more )
Specimen
1e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb mesenchyme |
Present |
Regionally restricted |
1e Hox-5.2
|
Weak expression in the posterior half of the forelimb tip.
|
TS20: hindlimb mesenchyme |
Present |
Regionally restricted |
1e Hox-5.2
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Weak expression in the posterior half of the forelimb tip.
|
Specimen
1f:
embryonic day 14.5
(more )
Specimen
1f:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: foot mesenchyme |
Absent |
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1f Hox-5.2
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No expression in mesenchymal cells surrounding the phalangeal cartilage anlage.
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Specimen
2a,b:
embryonic day 9.75
(more )
Specimen
2a,b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.75
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3a:
embryonic day 10.0
(more )
Specimen
3a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
3a Hox-5.2
|
Expression in posterior mesoderm; no expression in the most anterior mesoderm. Weak expression in the posterior half of the forelimb tip.
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Specimen
3b:
embryonic day 10.0
(more )
Specimen
3b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
3b Hox-5.2
|
Expression in a wide part of the bud, with no expression in the very anterior margin.
|
Specimen
3c:
embryonic day 10.0
(more )
Specimen
3c:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
3c Hox-5.2
|
Expression in a wide part of the bud, with no expression in the very anterior margin.
|
Specimen
3d:
embryonic day 10.0
(more )
Specimen
3d:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
3d Hox-5.2
|
Expression in a wide part of the bud, with no expression in the very anterior margin.
|
Specimen
3e:
embryonic day 10.0
(more )
Specimen
3e:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: forelimb bud mesenchyme |
Present |
Regionally restricted |
3e Hox-5.2
|
Gradual decrease in expression towards more distal regions.
|
Specimen
4a:
embryonic day 10.5
(more )
Specimen
4a:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
4a Hox-5.2
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Proximally, expression in the whole mesoderm, but stronger in the central condensation.
|
Specimen
4b:
embryonic day 12.5
(more )
Specimen
4b:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|