Probe:	pELF2E
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Expression analysis was performed using three different probes. Probes pELF2H (MGI:4441591) or pELF2I (MGI:4441592) may have been used. Authors report that all three probes gave the same expression pattern.

Structure	Level	Pattern	Image	Note
TS13: future prosencephalon	Weak	Regionally restricted	7A	Weak expression within restricted regions of forebrain.
TS13: future hindbrain	Strong	Regionally restricted	7A	Strongest expression in the anterior hindbrain at the level of rhombomeres 1,2,4 and 6; weak or absent at the levels of rhombomeres 3 and 5.
TS13: branchial arch	Strong		7A
TS13: somite	Weak		7A	Weak expression in segmented mesoderm.
TS13: trunk paraxial mesenchyme	Strong	Regionally restricted	7A	Two intense bands in the region of somite formation at the boundary between somites and presomitic mesoderm. Anterior band in or near the most recently formed somite; posterior band in or near the somite undergoing condensation.
TS13: trunk unsegmented mesenchyme	Absent		7A	No expression in more posterior regions of of presomitic mesoderm near the caudal end of embryo.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Weak		7C
TS15: future midbrain	Weak	Regionally restricted	7C	Particularly in anterior end of the midbrain.
TS15: future hindbrain	Strong		7C
TS15: somite	Weak	Regionally restricted	7C	Weak expression in bands in more anterior somites of segmented mesoderm.
TS15: paraxial mesenchyme	Strong	Regionally restricted	7C	Two bands of expression near the border between somites and presomitic mesoderm. Anterior band is narrower and more intense.

Structure	Level	Pattern	Image	Note
TS17: forebrain	Present		7E
TS17: hindbrain	Weak		7E	Reduced expression in the hindbrain compared with earlier stages.
TS17: branchial arch	Present		7E
TS17: forelimb bud	Present		7E
TS17: tail paraxial mesenchyme	Strong	Regionally restricted	7E	Strongest expression as bands in the region of somitogenesis.

Structure	Level	Pattern	Image	Note
TS18: forebrain	Present		7G
TS18: head	Present	Regionally restricted	7G	Expression near the optic cup.
TS18: branchial arch	Present		7G
TS18: forelimb bud	Present		7G
TS18: hindlimb bud	Present		7G