Gene Expression Data
Assay Details
Assay
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Reference:
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J:9026
Nomura S, et al., Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization. J Cell Biol. 1988 Feb;106(2):441-50
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4441386
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Gene symbol:
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Spp1
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Gene name:
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secreted phosphoprotein 1
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Probe:
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2ar cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
not shown:
embryonic day 6.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 6.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Present |
Regionally restricted |
|
Expression localized in the central zone and metrial triangle.
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Specimen
2a,e:
embryonic day 7.5
(more )
Specimen
2a,e:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 7.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Present |
Regionally restricted |
2a/e
|
Expression in metrial gland cells in the central zone.
|
Specimen
not shown:
embryonic day 8.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 8.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Present |
Regionally restricted |
|
|
Specimen
2b,f:
embryonic day 9.5
(more )
Specimen
2b,f:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Present |
Regionally restricted |
2b/f
|
Expression in metrial gland cells in the central zone.
|
Specimen
2d,h - decidua:
embryonic day 10.5
(more )
Specimen
2d,h - decidua:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua |
Present |
Regionally restricted |
2d/h
|
Expression in metrial gland cells in the central zone.
|
Specimen
2d,h - embryo:
embryonic day 10.5
(more )
Specimen
2d,h - embryo:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3a:
embryonic day 14.5
(more )
Specimen
3a:
(close )
Genetic Background:
|
CBA
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua basalis |
Present |
Regionally restricted |
3a
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Expression in a region containing many cells with intense PAS staining.
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Specimen
3d:
embryonic day 14.5
(more )
Specimen
3d:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua basalis |
Present |
Regionally restricted |
3d/e
|
Expression specifically associated with cells containing PAS-positive granules.
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Specimen
3e:
embryonic day 14.5
(more )
Specimen
3e:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: decidua basalis |
Present |
Regionally restricted |
|
Expression in a metrial gland cell with PAS-positive granules.
|
Specimen
3f,g:
embryonic day 14.5
(more )
Specimen
3f,g:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: humerus cartilage condensation |
Present |
Regionally restricted |
3f/g
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Expression in central region of humerus in small, fibroblast-like cells internal to the periosteum and its associated matrix.
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Specimen
3j:
embryonic day 15.5
(more )
Specimen
3j:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: forelimb skeleton |
Present |
Regionally restricted |
3j
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Expression associated with cells in the newly excavated marrow region.
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Specimen
3l:
embryonic day 15.5
(more )
Specimen
3l:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: skeleton |
Present |
Regionally restricted |
3l
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Expression in the central marrow region where endochondral ossification is being initiated.
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TS23: cartilage |
Absent |
|
3l
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No expression in hypertrophic cartilage.
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Specimen
3n:
embryonic day 17.5
(more )
Specimen
3n:
(close )
Genetic Background:
|
CBA
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Age:
|
embryonic day 17.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: alveolar sulcus |
Present |
Regionally restricted |
3n
|
Expression in alveolar bone in the jaw in individual cells closely associated with bone matrix.
|
Specimen
not shown:
embryonic day 14.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5c,d:
embryonic day 18.5
(more )
Specimen
5c,d:
(close )
Genetic Background:
|
CBA
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: renal tubule |
Present |
Regionally restricted |
5c/d
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Increase in number of expressing tubules.
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Specimen
5a,b:
postnatal day 3
(more )
Specimen
5a,b:
(close )
Genetic Background:
|
CBA
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Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
5e,f:
postnatal day 3
(more )
Specimen
5e,f:
(close )
Genetic Background:
|
CBA
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown:
embryonic day 16.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: ear |
Strong |
Regionally restricted |
|
High levels of expression in localized regions.
|
Specimen
not shown:
embryonic day 17.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: ear |
Strong |
Regionally restricted |
|
High levels of expression in localized regions.
|
Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
postnatal adult
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown:
postnatal adult
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown:
postnatal adult
(more )
Note: Specimen is from a pregnant female.
Specimen
not shown:
(close )
Genetic Background:
|
CBA
|
Age:
|
postnatal adult
|
Sex:
|
Female
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Specimen is from a pregnant female.
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: adrenal cortex |
Present |
Regionally restricted |
|
Small clusters of cells, near junction of zona fasciculata and reticularis.
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