Igf1 Western blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:148934 Salehi Z, et al., Insulin-like growth factor-1 and insulin-like growth factor binding proteins in cerebrospinal fluid during the development of mouse embryos. J Clin Neurosci. 2009 Jul;16(7):950-3
Assay type:	Western blot
MGI Accession ID:	MGI:4440743
Gene symbol:	Igf1
Gene name:	insulin-like growth factor 1

Antibody:	IGF-1 Antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Cerebrospinal fluid was aspirated from the lateral ventricle of embryonic mice using micromanipulation. An image analyzer was used to determine the relative intensities of the gels from repeated experiments (n = 26 for each time) which is graphed in Figure 3.

Results

Image: 2

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	7.65 kDa	Genetic Background	Mutant Allele(s)	Sex	Note
E12	E12.5 (a)	TS20: body fluid or substance	Present	BALB/c		Not Specified	Cerebrospinal fluid
E14	E14.5 (a)	TS22: body fluid or substance	Present	BALB/c		Not Specified	Cerebrospinal fluid
E15	E15.5 (a)	TS23: body fluid or substance	Present (b)	BALB/c		Not Specified	Cerebrospinal fluid
E16	E16.5 (a)	TS24: body fluid or substance	Present (c)	BALB/c		Not Specified	Cerebrospinal fluid
E17	E17.5 (a)	TS25: body fluid or substance	Present (c)	BALB/c		Not Specified	Cerebrospinal fluid
E18	E18.5 (a)	TS26: body fluid or substance	Present (c)	BALB/c		Not Specified	Cerebrospinal fluid
E19	E19.5 (a)	TS26: body fluid or substance	Present (d)	BALB/c		Not Specified	Cerebrospinal fluid
E20	E20.5 (a)	TS26: body fluid or substance	Present (d)	BALB/c		Not Specified	Cerebrospinal fluid
E21	E21.5 (a)	TS26: body fluid or substance	Present (d)	BALB/c		Not Specified	Cerebrospinal fluid

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) The amounts decreased from days E12 to E15.
(c) There was an increase in concentration on days E16 to E18.
(d) Expression levels decreased.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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