Probe:	c-kit cDNA4
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted	2A/B	Expression in the middle of the maternal placenta.
TS11: embryo	Absent		2A/B

Structure	Level	Pattern	Image	Note
TS13: chorioallantoic placenta	Present		2C/D
TS13: yolk sac	Ambiguous		2C/D	Authors state that expression in the yolk sac was less clear.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		2E
TS13: neural tube	Present		2E
TS13: inner ear	Present	Regionally restricted	2E	Expression in the otic vesicle
TS13: 1st branchial arch	Present	Regionally restricted	2E	Expression in or under the surface of the mandibular arch.
TS13: heart	Absent		2E

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present		3A/B

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present		3C/D
TS14: 1st branchial arch ectoderm	Present		3C/D	Expression in the surface ectoderm of the head corresponding to the first two branchial arches.
TS14: 2nd branchial arch ectoderm	Present		3C/D	Expression in the surface ectoderm of the head corresponding to the first two branchial arches.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Absent		3E/F
TS14: 1st branchial arch ectoderm	Present		3E/F	Expression in the surface ectoderm of the head corresponding to the first two branchial arches.
TS14: 2nd branchial arch ectoderm	Present		3E/F	Expression in the surface ectoderm of the head corresponding to the first two branchial arches.

Structure	Level	Pattern	Image	Note
TS13: branchial arch ectoderm	Present	Regionally restricted	3G/H	Expression in the surface ectoderm of the head corresponding to the first two branchial arches.
TS13: somite	Absent		3G/H

Structure	Level	Pattern	Image	Note
TS15: branchial arch ectoderm	Present		4A/B	Expression in the surface ectoderm of the branchial arches.
TS15: branchial pouch	Present	Regionally restricted	4A/B	Expression in the endoderm of the branchial pouches, concentrated at the posterior surface of the branchial pouches.
TS15: heart	Absent		4A/B
TS15: vertebral pre-cartilage condensation	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS15: branchial arch ectoderm	Present		4D/E	Expression in the surface ectoderm of the branchial arches.
TS15: branchial arch endoderm	Present	Regionally restricted	4D/E	Expression in the endoderm of the branchial pouches, concentrated at the posterior surface of the branchial pouches.
TS15: branchial pouch	Present	Regionally restricted	4D/E	Expression in the endoderm of the branchial pouches, concentrated at the posterior surface of the branchial pouches.
TS15: head	Present	Regionally restricted	4D/E	Expression on the ventrolateral surface of the frontal bulge of the head - probably represents expression in the developing olfactory epithelium.

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from somatopleure	Strong	Regionally restricted	4F/G	Strong expression in somatic and splanchnic mesoderm surrounding the aorta.
TS15: mesenchyme derived from splanchnopleure	Strong	Regionally restricted	4F/G	Strong expression in somatic and splanchnic mesoderm surrounding the aorta.
TS15: future hindbrain	Present		4F/G
TS15: heart	Absent		4F/G
TS15: somite	Absent		4F/G

Structure	Level	Pattern	Image	Note
TS15: mesenchyme derived from somatopleure	Strong	Regionally restricted	4H/I	Strong expression in somatic and splanchnic mesoderm surrounding the aorta.
TS15: mesenchyme derived from splanchnopleure	Strong	Regionally restricted	4H/I	Strong expression in somatic and splanchnic mesoderm surrounding the aorta.
TS15: future hindbrain	Present		4H/I
TS15: future spinal cord	Present		4H/I
TS15: forelimb bud	Weak		4H/I
TS15: somite	Absent		4H/I

Structure	Level	Pattern	Image	Note
TS15: yolk sac mesenchyme	Moderate	Regionally restricted	5A/B	Expression in most cells of the blood islands.

Structure	Level	Pattern	Image	Note
TS20: forebrain marginal layer	Present		6A/B
TS20: spinal cord	Present	Regionally restricted	6A/B	Expression mostly in the dorsal portion of the spinal cord.
TS20: liver parenchyma	Present		6A/B
TS20: liver sinusoid	Weak	Regionally restricted	6A/B	Much less specific expression in more mature blood cells within liver sinuses.
TS20: stomach	Strong	Regionally restricted	6A/B	Strong expression in the muscular layer; somewhat weaker in the endothelium.
TS20: esophagus	Present	Regionally restricted	6A/B	Expression in the endothelium
TS20: oral region	Present	Regionally restricted	6A/B	Expression in the endothelium
TS20: pharynx	Present	Regionally restricted	6A/B	Expression in the endothelium
TS20: hindgut epithelium	Present	Regionally restricted	6A/B	Expression in colon and rectum endothelium.
TS20: skin	Strong	Spotted	6A/B	Punctate expression in subepidermal spaces in the nose and tail.`

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6D/E	Expression mostly in the dorsal portion of the spinal cord.
TS20: liver parenchyma	Present		6D/E
TS20: liver sinusoid	Weak	Regionally restricted	6D/E	Much less specific expression in more mature blood cells within liver sinuses.
TS20: genital tubercle	Strong		6D/E	Authors report strong expression in the genital ridge.
TS20: hindlimb	Weak		6D/E
TS20: intestine	Strong	Scattered	6D/E	Strong expression in the muscular layer; somewhat weaker in the endothelium.
TS20: stomach	Strong	Scattered	6D/E	Strong expression in the muscular layer; somewhat weaker in the endothelium.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present		6F
TS20: liver parenchyma	Present		6F
TS20: spinal cord	Present		6F
TS20: liver sinusoid	Weak		6F
TS20: genital tubercle	Strong		6F	Authors report strong expression in the genital ridge.
TS20: intestine	Strong	Regionally restricted	6F	Strong expression in the muscular layer; somewhat weaker in the endothelium.
TS20: stomach	Strong	Regionally restricted	6F	Strong expression in the muscular layer; somewhat weaker in the endothelium.

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Present

Structure	Level	Pattern	Image	Note
TS22: nasal cavity epithelium	Present		7B
TS22: upper jaw incisor dental papilla	Present		7B
TS22: upper jaw incisor enamel organ	Present		7B
TS22: skin	Present	Spotted	7B	Punctate expression in subepidermal spaces in the nose and tail.

Structure	Level	Pattern	Image	Note
TS22: pharynx epithelium	Strong		7C
TS22: thymus primordium	Strong		7C
TS22: tongue intrinsic muscle	Strong		7C
TS22: thyroid gland	Strong	Regionally restricted	7C	Strong exression in the thyroid duct and in structures surrounding the prospective thyroid cartilage.

Structure	Level	Pattern	Image	Note
TS22: vibrissa	Present	Regionally restricted	7D	Expression in cells in the root of sensory hairs in the snout.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	7E	Expression in cells in the subepidermal space of the face.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Spotted	7F/G	Punctate expression in subepidermal spaces of the buccal area of the head, most likely tissue mast cells and/or melanocyte precursors.

Structure	Level	Pattern	Image	Note
TS22: hindbrain	Present
TS22: telencephalon marginal layer	Present
TS22: spinal cord	Present	Regionally restricted		Expression in the dorsal part of the spinal cord.
TS22: gut	Present
TS22: liver	Present
TS22: lung	Present
TS22: pancreas	Present

Structure	Level	Pattern	Image	Note
TS25: cerebellum	Present
TS25: hindbrain	Present
TS25: telencephalon marginal layer	Present
TS25: spinal cord	Present	Regionally restricted		Expression in the dorsal part of the spinal cord.
TS25: gut	Present
TS25: liver	Present
TS25: lung	Present
TS25: pancreas	Present

Structure	Level	Pattern	Image	Note
TS20: genital tubercle	Present	Regionally restricted	8B	Expression in the genital ridge.
TS20: primordial germ cell of gonad	Present		8B

Structure	Level	Pattern	Image	Note
TS28: ovary antral follicle	Present		9A/B
TS28: ovary primary follicle	Present		9A/B
TS28: oocyte	Strong		9A/B	Highest expression in the oocytes themselves.

Structure	Level	Pattern	Image	Note
TS28: ovary mature follicle	Present		9C/D
TS28: oocyte	Strong		9C/D	Highest expression in the oocytes themselves.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: Leydig cell	Leydig cell	Present	Regionally restricted	9E/F
TS28: Sertoli cell	Sertoli cell	Absent		9E/F
TS28: spermatogonium		Absent		9E/F