Gene Expression Data
Assay Details
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Assay
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Reference:
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J:157526
Gestreau C, et al., Task2 potassium channels set central respiratory CO2 and O2 sensitivity. Proc Natl Acad Sci U S A. 2010 Feb 2;107(5):2325-30
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:4438119
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Gene symbol:
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Kcnk5
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Gene name:
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potassium channel, subfamily K, member 5
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1A:
(close  )
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Genetic Background:
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B6.129P2-Kcnk5Gt(KST024)Byg
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5Gt(KST024)Byg
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: brainstem |
Present |
Regionally restricted |
1A
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Authors report that expression was restricted to very few brainstem regions and was absent in other brain regions.
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| TS28: medulla oblongata |
Present |
Regionally restricted |
1A
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Expression at the ventral medullary surface surface (VMS) around the facial motor (VII) nucleus, in the retrotrapezoid nucleus (RTN) and parafacial respiratory group (pfRG). No expression in the medial parapyramidal region.
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Specimen
1B:
(close )
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Genetic Background:
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B6.129P2-Kcnk5Gt(KST024)Byg
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5Gt(KST024)Byg
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: midbrain |
Present |
Regionally restricted |
1B
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Expression in the dorsal raphe (DR) nucleus and in the intermediate nucleus of the lateral lemniscus (ILL).
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Specimen
not shown:
(close )
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Genetic Background:
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B6.129P2-Kcnk5Gt(KST024)Byg
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5Gt(KST024)Byg
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: inferior colliculus |
Weak |
Regionally restricted |
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Sparse labeling in the caudal inferior colliculus, in the central nucleus of the inferior colliculus (CIC).
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Specimen
1C:
(close )
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Genetic Background:
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B6.129P2-Kcnk5Gt(KST024)Byg
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5Gt(KST024)Byg
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pons |
Present |
Regionally restricted |
1C
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Expression in the rostral pons.
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| TS28: superior olivary nucleus |
Present |
Regionally restricted |
1C
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Expression in the lateral superior olive (LSO).
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Specimen
1D:
(close )
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Genetic Background:
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B6.129P2-Kcnk5Gt(KST024)Byg
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5Gt(KST024)Byg
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: pons |
Present |
Regionally restricted |
1D
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In the caudal pons, espression at the ventral surface in the parvicellular reticular formation pars alpha (PCRtA).
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Specimen
1E:
(close )
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Genetic Background:
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B6.129P2-Kcnk5Gt(KST024)Byg
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5Gt(KST024)Byg
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: medulla oblongata |
Present |
Regionally restricted |
1E
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Expression at the ventral medullary surface (VMS) at the caudal end of the facial motor (VII) nucleus.
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Specimen
not shown:
(close )
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Genetic Background:
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B6.129P2-Kcnk5Gt(KST024)Byg
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5Gt(KST024)Byg
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spinal cord |
Absent |
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No expression in the cervical spinal cord.
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Specimen
2A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * 129S2/SvPas * C57BL/6
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Mutant Allele(s):
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Kcnk5Gt(KST024)Byg/Kcnk5+
Phox2btm2Jbr/Phox2b+
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: brain |
Present |
Regionally restricted |
2B
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Authors state that expression is specifically lost in retrotrapezoid nucleus neurons.
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| TS23: brain meninges |
Present |
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2B
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Analysis Tools
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