Summary | Transgene origin | Transgene description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo
Name:	transgene insertion 1, Andrew Wolfe
MGI ID:	MGI:4437221
Synonyms:	GnRH-CRETeR
Transgene:	Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo  Location: unknown
Alliance:	Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo page

Strain of Origin:

Not Specified

Transgene Type:    Transgenic (Inducible, Inserted expressed sequence, Recombinase) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Tg(Gnrh1-tetO-cre,CMV-tetR)1Awo expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Gnrh1 (MGI:95789)   Mutation details: A -3446 bp fragment of the mouse Gnrh1 promoter was fused upstream of cre recombinase. The proximal region of the Gnrh1 promoter was modified to insert two Tet operators (tetO2). In series, the transgene also contains the CMV promoter regulating the Tet repressor gene (tetR). The rabbit beta-globin intron II is inserted between the promoter and the gene to enhance expression of the tetR gene. In absence of doxycycline, the tet repressor protein binds to the tet operators, blocking transcription. Induction by doxycycline releases the repression, permitting transcription of cre recombinase. Four founders were generated and individually analyzed for function and specificity. A representative line no. 1 was created. (J:157600)

Activity:	Tissue activity of this recombinase allele
Driver:	Gnrh1 (mouse) Summary of all recombinase alleles driven by Gnrh1. Comparative matrix view of recombinase activities Alliance mouse Gnrh1 gene page

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:157600 Wolfe A, et al., Temporal and spatial regulation of CRE recombinase expression in gonadotrophin-releasing hormone neurones in the mouse. J Neuroendocrinol. 2008 Jul;20(7):909-16
All:	1 reference(s)