Gene Expression Data
Assay Details
Assay
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Reference:
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J:38581
Sommer L, et al., neurogenins, a novel family of atonal-related bHLH transcription factors, are putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS. Mol Cell Neurosci. 1996;8(4):221-41
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4437108
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Gene symbol:
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Neurog3
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Gene name:
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neurogenin 3
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Probe:
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ngn3 cDNA
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 9.0
(more )
Specimen
not shown:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS14: neural tube |
Strong |
Regionally restricted |
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Strong expression in a very restricted region of the spinal cord, just dorsal to the floor plate.
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Specimen
3G:
embryonic day 10.5
(more )
Note: section through trunk at level of forelimb
Specimen
3G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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section through trunk at level of forelimb
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Specimen
3H:
embryonic day 12.0
(more )
Note: section through trunk at level of forelimb
Specimen
3H:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
section through trunk at level of forelimb
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
3H
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Strong expression in a very restricted region of the spinal cord, just dorsal to the floor plate. A weaker, more dorsally located, domain of expression, near the midpoint of the spinal cord.
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TS20: trunk mesenchyme |
Absent |
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3H
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No expression in trunk neural crest or its derivatives.
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Specimen
not shown:
embryonic day 14.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 14.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: spinal cord |
Weak |
Regionally restricted |
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Expression in a few cells in a region of the spinal cord, just dorsal to the floor plate.
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TS22: trunk mesenchyme |
Absent |
|
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No expression in trunk neural crest or its derivatives.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Present |
Regionally restricted |
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Expression in the hypothalamic region.
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Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: hypothalamus |
Present |
|
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Expression mostly limited to the hypothalamic area.
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Specimen
9A:
embryonic day 12.0
(more )
Specimen
9A:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: diencephalon |
Strong |
Regionally restricted |
9A
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Strong expression in the ventricular zone dorsal to the pituitary.
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Specimen
9C:
embryonic day 12.0
(more )
Specimen
9C:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS20: pancreas |
Strong |
Regionally restricted |
9C
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Strong expression in a few cells in the pancreatic primordium.
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Specimen
9E:
embryonic day 14.0
(more )
Specimen
9E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
Paraformaldehyde
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: pancreas |
Strong |
Regionally restricted |
9E
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Stronger expression in a larger number of cells in the pancreatic primordium.
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