Probe:	ngn2 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS13: neural tube	Present	Regionally restricted	3B	Expression in ventral spinal cord.
TS13: migrating neural crest	Present		3B	Expression in cells dorso-lateral to the neural tube. Authors report that expression is in neural crest cells early in migration.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Strong	Regionally restricted	3D	Strong expression in the ventricular zone of the ventral neural tube. Expression in a limited area of the dorsal neural tube near the roof plate.
TS17: dorsal root ganglion	Weak	Regionally restricted	3D	Weak expression in subsets of cells in the dorsal lateral margins of the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS17: enteric nervous system	Absent			No expression in sympathetic or enteric ganglia.
TS17: sympathetic nervous system	Absent			No expression in sympathetic or enteric ganglia.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	3F	Expression throughout the dorso-ventral axis of the spinal cord.
TS20: spinal cord ventricular layer	Present	Regionally restricted	3F	Expression in both medial and lateral parts of the ventricular zone.
TS20: dorsal root ganglion	Absent		3F

Structure	Level	Pattern	Image	Note
TS14: future spinal cord	Present	Regionally restricted	4B	Expression in 2 longitudinal stripes that define dorsal and ventral domains of expression in the spinal cord.
TS14: migrating neural crest	Present	Regionally restricted	4B	Expression in a third longitudinal stripe located ventro-lateral to the dorsal stripe, consistent with expression in early emigrating neural crest cells.
TS14: embryo ectoderm	Strong	Regionally restricted	4B	Strong expression in the epibranchial placodes.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord	Present	Regionally restricted	4D	Expression in 2 longitudinal stripes that define dorsal and ventral domains of expression in the spinal cord.
TS14: trunk mesenchyme derived from neural crest	Present	Regionally restricted	4D	Expression in an antero-posterior stripe located between the stripe of its expression in the dorsal neural tube and ventral neural tube, declining anteriorly.
TS14: neural crest	Trace	Regionally restricted	4D	Domain of strong expression in neural crest declines sharply within the cervical region. Weak or undetectable expression in the cervical region.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Absent			Low or undetectable expression in migrating neural crest cells in the cervical region.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Absent		5B	No expresssion in migrating neural crest at the cervical level.

Structure	Level	Pattern	Image	Note
TS13: migrating neural crest	Present		5D	Expression in migrating neural crest at the the thoracolumbar level.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present	Regionally restricted	5F	No expression in forming sensory ganglia at cervical levels.

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present	Regionally restricted	5H	Expression in forming sensory ganglia at thoracolumbar levels.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present	Regionally restricted	5J	No expression at cervical levels.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present	Regionally restricted	5L	Expression in dorsal root ganglia at the level of the hindlimb.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Strong	Regionally restricted	6B	High levels of expression in the cortical neuroepithelium.
TS20: forebrain ventricular layer	Present		6B	Expression in the forebrain, restricted to the ventricular zone.
TS20: diencephalon	Strong	Regionally restricted	6B	High levels of expression in a portion of the diencephalon.
TS20: corpus striatum	Absent		6B

Structure	Level	Pattern	Image	Note
TS20: hindbrain ventricular layer	Present		6E

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted		Expression in medial areas of the dorsal thalamus.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	7B	Expression in lateral areas of the dorsal thalamus.
TS20: diencephalon	Present	Regionally restricted	7B	Expression in the subthalamus.
TS20: 4th ventricle	Present	Regionally restricted	7B	Expression in the neuroepithelium lining the lateral ventricle, aqueduct and 4th ventricle.
TS20: lateral ventricle	Present	Regionally restricted	7B	Expression in the neuroepithelium lining the lateral ventricle, aqueduct and 4th ventricle.
TS20: mesencephalic vesicle	Present	Regionally restricted	7B	Expression in the neuroepithelium lining the lateral ventricle, aqueduct and 4th ventricle.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present	Regionally restricted	7D	Expression in the posterior tegmental epithelium.
TS20: diencephalon	Present	Regionally restricted	7D	Expression in the subthalamus.
TS20: mesencephalic vesicle	Present	Regionally restricted	7D	Expression in the neuroepithelium lining the aqueduct.

Structure	Level	Pattern	Image	Note
TS20: trigeminal ganglion	Absent		8B

Structure	Level	Pattern	Image	Note
TS22: olfactory epithelium	Present		8E

Structure	Level	Pattern	Image	Note
TS22: retina	Present		8H

Structure	Level	Pattern	Image	Note
TS17: glossopharyngeal ganglion	Present		8K

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Present		8N
TS17: acoustic ganglion	Absent		8N
TS17: otocyst	Absent		8N

Structure	Level	Pattern	Image	Note
TS20: diencephalon ventricular layer	Present	Regionally restricted		No expression in the ventricular zone dorsal to the pituitary.

Structure	Level	Pattern	Image	Note
TS20: pancreas	Absent			No expression in the pancreatic primordium.