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Gene Expression Data
Assay Details
Assay
Reference: J:87352 Sakamoto J, et al., Predominant expression of Sir2alpha, an NAD-dependent histone deacetylase, in the embryonic mouse heart and brain. FEBS Lett. 2004 Jan 2;556(1-3):281-6
Assay type: Northern blot
MGI Accession ID: MGI:4431186
Gene symbol: Sirt1
Gene name: sirtuin 1
Probe: Sir2alpha cDNA
Probe preparation: Antisense labelled with P32 RNA
Visualized with: Autoradiography
Assay notes: Densitometric analysis of blots is shown in Fig. 1D.
Results Image: 1C
 Sample Information Bands Other Sample Information
Lane AgeStructure 5 kb Amount Genetic BackgroundMutant Allele(s)Sex
E4.5 E4.5 (a) TS6: embryo Present (b) 20 µg; total RNA Not Specified Not Specified
E5.5 E5.5 (a) TS8: embryo Present 20 µg; total RNA Not Specified Not Specified
E6.5 E6.5 (a) TS9: embryo Present 20 µg; total RNA Not Specified Not Specified
E7.5 E7.5 (a) TS11: embryo Present 20 µg; total RNA Not Specified Not Specified
E8.5 E8.5 (a) TS13: embryo Present 20 µg; total RNA Not Specified Not Specified
E9.5 E9.5 (a) TS15: embryo Present 20 µg; total RNA Not Specified Not Specified
E10.5 E10.5 (a) TS17: embryo Present 20 µg; total RNA Not Specified Not Specified
E11.5 E11.5 (a) TS19: embryo Present 20 µg; total RNA Not Specified Not Specified
E12.5 E12.5 (a) TS20: embryo Present 20 µg; total RNA Not Specified Not Specified
E13.5 E13.5 (a) TS21: embryo Present 20 µg; total RNA Not Specified Not Specified
E14.5 E14.5 (a) TS22: embryo Present 20 µg; total RNA Not Specified Not Specified
E15.5 E15.5 (a) TS23: embryo Present 20 µg; total RNA Not Specified Not Specified
E16.5 E16.5 (a) TS24: embryo Present 20 µg; total RNA Not Specified Not Specified
E17.5 E17.5 (a) TS25: embryo Present 20 µg; total RNA Not Specified Not Specified
E18.5 E18.5 (a) TS26: embryo Present (c) 20 µg; total RNA Not Specified Not Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) The highest expression levels were found in early-stage embryos.
(c) The expression level in E18.5 had decreased by 60% compared with that on E4.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory