Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:19211
Conlon FL, et al., A primary requirement for nodal in the formation and maintenance of the primitive streak in the mouse. Development. 1994 Jul;120(7):1919-28
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4431067
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Gene symbol:
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Gsc
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Gene name:
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goosecoid homeobox
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Probe:
|
Probe 2
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Probe preparation:
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RNA
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Visualized with:
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Autoradiography
|
|
Results
|
Specimen
not shown:
embryonic day 5.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 5.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3D:
embryonic day 6.5
(more )
Note: Early streak stage.
Specimen
3D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 6.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Early streak stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS9: embryo mesoderm |
Strong |
Regionally restricted |
3D
|
High levels of expression confined to anterior mesoderm; initially expressed in the mesoderm at the anterior-most region of the streak.
|
TS9: primitive endoderm |
Strong |
Regionally restricted |
3D
|
High levels of expression in the visceral endoderm anterior to the streak.
|
Specimen
not shown:
embryonic day 7.0
(more )
Note: Mid-streak stage.
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Mid-streak stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo mesoderm |
Strong |
Regionally restricted |
|
High expression in mesodermal cells in the anterior region of the streakk.
|
TS10: embryo endoderm |
Strong |
Regionally restricted |
|
High expression in the endoderm anterior to the streak.
|
Specimen
not shown:
embryonic day 7.5
(more )
Specimen
not shown:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
|