Gene Expression Data
Assay Details
Assay
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Reference:
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J:2250
Aizawa T, et al., Expression of necdin, an embryonal carcinoma-derived nuclear protein, in developing mouse brain. Brain Res Dev Brain Res. 1992 Aug 21;68(2):265-74
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:4430918
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Gene symbol:
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Ndn
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Gene name:
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necdin, MAGE family member
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Antibody:
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antibody C2
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
6B:
embryonic day 10.0
(more )
Specimen
6B:
(close )
Genetic Background:
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DDY
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Age:
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embryonic day 10.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6D:
embryonic day 15.0
(more )
Specimen
6D:
(close )
Genetic Background:
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DDY
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Age:
|
embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: hypothalamus |
Present |
Regionally restricted |
6D
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In the anterior hypothalamic neuroepithelium, expression in 50% of nuclei; expression absent in other nuclei.
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Specimen
6F:
embryonic day 18.0
(more )
Specimen
6F:
(close )
Genetic Background:
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DDY
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Age:
|
embryonic day 18.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: hypothalamus |
Present |
Regionally restricted |
6F
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In the anterior hypothalamic area, expression in most or all nuclei; intensity of expression varied between nuclei.
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Specimen
6H:
postnatal day 2
(more )
Specimen
6H:
(close )
Genetic Background:
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DDY
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Age:
|
postnatal day 2
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: hypothalamus periventricular zone |
Present |
Regionally restricted |
6H
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In the paraventricular region of the anterior hypothalamic area, expression in nuclei; little or no expression in ependymal cells.
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Specimen
7B:
postnatal day 33
(more )
Specimen
7B:
(close )
Genetic Background:
|
DDY
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Age:
|
postnatal day 33
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: parietal cortex |
Strong |
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7B
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Intense expression in most of the nuclei.
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Specimen
7D:
postnatal day 33
(more )
Specimen
7D:
(close )
Genetic Background:
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DDY
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Age:
|
postnatal day 33
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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