Gene Expression Data
Assay Details
Assay
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Reference:
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J:2111
Sarthy PV, et al., Developmental expression of the glial fibrillary acidic protein (GFAP) gene in the mouse retina. Cell Mol Neurobiol. 1991 Dec;11(6):623-37
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4430850
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Gene symbol:
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Gfap
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Gene name:
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glial fibrillary acidic protein
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Probe:
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Gfap cDNA2
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Probe preparation:
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labelled with S35 DNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2A,B:
embryonic day 15.0
(more )
Specimen
2A,B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: retina |
Absent |
|
2B
,
2A
|
|
Specimen
not shown:
embryonic day 16.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: optic nerve |
Present |
|
|
Expression first observed in the optic nerve.
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Specimen
not shown:
embryonic day 17.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 17.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: optic nerve |
Present |
|
|
Expression first observed in the optic nerve.
|
Specimen
2C,D:
embryonic day 20.0
(more )
Specimen
2C,D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 20.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: optic nerve |
Present |
Regionally restricted |
2C
,
2D
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Expression in a large number of cells in the optic nerve head.
|
TS26: retina |
Absent |
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2C
,
2D
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Specimen
3A,B:
postnatal day 1
(more )
Specimen
3A,B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 1
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Cryosection
|
Specimen
3C,D:
postnatal day 3
(more )
Specimen
3C,D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 3
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: optic disc |
Strong |
|
3D
,
3C
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The number of expressing cells and intensity of expression increased dramatically in the optic nerve of postnatal retinas.
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TS27: retina |
Present |
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3C
,
3D
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Specimen
3E,F:
postnatal day 5
(more )
Specimen
3E,F:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: optic nerve |
Strong |
|
3F
,
3E
|
The number of expressing cells and intensity of expression increased dramatically in the optic nerve of postnatal retinas.
|
TS28: retina |
Present |
|
3E
,
3F
|
|
Specimen
3G,H:
postnatal day 7
(more )
Specimen
3G,H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: optic nerve |
Strong |
|
3H
,
3G
|
The number of expressing cells and intensity of expression increased dramatically in the optic nerve of postnatal retinas.
|
TS28: retina |
Present |
|
3G
,
3H
|
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Specimen
4A:
postnatal day 1
(more )
Specimen
4A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: optic disc |
Present |
Regionally restricted |
4A
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Expression in the optic nerve head region of the retina. Expression in some cells at the boundary of the optic nerve head and the internal limiting membrane.
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Specimen
4B:
postnatal day 3
(more )
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: retina ganglion cell layer |
Present |
Regionally restricted |
4B
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Expression in the ganglion cell layer; expressing cells extended from the optic disc to about a third of the retina.
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Specimen
4C:
postnatal day 5
(more )
Specimen
4C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Present |
Regionally restricted |
4C
|
Expression in the ganglion cell layer of the central retina. Expression in cells in the central half of the retina.
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Specimen
4D:
postnatal day 5
(more )
Specimen
4D:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina |
Present |
Regionally restricted |
4D
|
No expression in the peripheral region of the retina.
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Specimen
4E:
postnatal day 7
(more )
Specimen
4E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Present |
Regionally restricted |
4E
|
Expression in the ganglion cell layer of the central retina.
|
Specimen
4F:
postnatal day 7
(more )
Specimen
4F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Present |
Regionally restricted |
4F
|
Expression in the ganglion cell layer of the peripheral retina. Expression in cells across the entire retina extending from the optic nerve head to the periphery.
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Specimen
4G:
postnatal day 10
(more )
Specimen
4G:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Strong |
Regionally restricted |
4G
|
Strong expression in the ganglion cell layer of the central retina.
|
Specimen
4H:
postnatal day 10
(more )
Specimen
4H:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: retina ganglion cell layer |
Strong |
Regionally restricted |
4H
|
Strong expression in the ganglion cell layer of the peripheral retina. Heavily labeled cells even in the far periphery close to the ciliary body.
|
Specimen
5A:
embryonic day 15.0
(more )
Specimen
5A:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5B:
postnatal day 4
(more )
Specimen
5B:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 4
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
5C,D:
postnatal adult
(more )
Specimen
5C,D:
(close )
Genetic Background:
|
Not Specified
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Age:
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postnatal adult
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: optic disc |
Present |
|
5C
,
5D
|
Expression in the optic nerve head region of the retina.
|
|