Summary | Transgene origin | Transgene description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Tg(Foxp3-EGFP/icre)1aJbs
Name:	transgene insertion 1a, Jeffrey Bluestone
MGI ID:	MGI:4430213
Transgene:	Tg(Foxp3-EGFP/icre)1aJbs  Location: unknown
Alliance:	Tg(Foxp3-EGFP/icre)1aJbs page

Strain of Origin:

NOD/ShiLtJ

Transgene Type:    Transgenic (Recombinase) Mutation:    Insertion   Tg(Foxp3-EGFP/icre)1aJbs expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Foxp3 (MGI:1891436)   Mutation details: An EGFP-h-Cre cDNA (GFP fused to a codon-optimized, "humanized" cre recombinase sequence) was inserted directly downstream of the Foxp3 ATG start codon by homologous recombination in a 188-kb C57BL/6 BAC clone (RP23-143D8) containing the entire Foxp3 gene. The purified BAC was injected into pronuclei of nonobese, diabetic mouse embryos. Several founders were generated. GFP expression and lack of functional Foxp3 protein, as indicated by inability of the transgene to rescue Foxp3 null mice were analyzed and line 1 was established. Functional cre activity was assayed by breeding mice to Gt(ROSA)26Sortm1(EYFP)Cos reporter mice, and high specificity of recombination was detected in the lymph nodes and spleen. This is the first of two lines derived from founder 1. Cre mediated recombination and GFP expression is greater than in line 1c. (J:138808)

Activity:	Tissue activity of this recombinase allele
Driver:	Foxp3 (mouse) Summary of all recombinase alleles driven by Foxp3. Alliance mouse Foxp3 gene page

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:138808 Zhou X, et al., Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity. J Exp Med. 2008 Sep 1;205(9):1983-91
All:	15 reference(s)