Hes6 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:156017 Yokoyama S, et al., A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58. Dev Cell. 2009 Dec;17(6):836-48
Assay type:	RNA in situ
MGI Accession ID:	MGI:4429930
Gene symbol:	Hes6
Gene name:	hairy and enhancer of split 6

Probe:	Hes6 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Results

Specimen Table 1: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Strong			Expression is present in the muscle-forming region.

Specimen S2: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: limb mesenchyme	Present		S2_Hes6 E9.5	Expression is present in the muscle region.

Specimen S2: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: limb mesenchyme	Present		S2_Hes6 E10.5	Expression is present in the progress zone of the distal mesenchyme, as well as in the muscle region.

Specimen S2: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: limb mesenchyme	Present		S2_Hes6 E11.5	Expression is present in the progress zone of the distal mesenchyme, as well as in the muscle region.

Specimen Table S4 - E9.5: embryonic day 9.5 (more

)

Structure	Level	Pattern	Note
TS15: limb mesenchyme	Strong	Regionally restricted	Staining is present in the distal limb.
TS15: trunk somite	Strong
TS15: neural tube	Strong	Non-Uniform	Staining is strong in the medial neural tube and moderate in the lateral neural tube.
TS15: dorsal root ganglion	Strong

Specimen Table S4 - E10.5: embryonic day 10.5 (more

)

Structure	Level	Pattern	Note
TS17: limb mesenchyme	Strong	Non-Uniform	Staining is strong in the progress zone and middle limb and moderate in the proximal limb.
TS17: trunk somite	Strong
TS17: 1st branchial arch maxillary component	Moderate		Staining is present in the maxillary process.
TS17: 1st branchial arch	Moderate		Staining is present in the mandibular arch.
TS17: 2nd branchial arch	Moderate		Staining is present in the hyoid arch.
TS17: eye	Moderate
TS17: nasal pit	Strong		Staining is present in the olfactory placode.
TS17: otocyst	Strong		Staining is present in the otic pit.
TS17: telencephalon	Strong
TS17: midbrain	Strong
TS17: hindbrain	Strong		Staining is present in the metencephalon.
TS17: neural tube	Strong	Non-Uniform	Staining is strong in the medial neural tube and moderate in the lateral neural tube.
TS17: dorsal root ganglion	Strong

Specimen Table S4 - E11.5: embryonic day 11.5 (more

)

Structure	Level	Pattern	Note
TS19: limb mesenchyme	Strong	Non-Uniform	Staining is strong in the progress zone and muscle and moderate in the proximal limb.
TS19: limb	Moderate	Regionally restricted	Staining is present in the antero-proximal region of the limb.
TS19: trunk somite	Strong
TS19: neural tube	Strong	Non-Uniform	Staining is strong in the medial neural tube and moderate in the lateral neural tube.
TS19: dorsal root ganglion	Strong

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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